IMMUNOLOGY PRACTICAL

Paper Code: 
24DZOL502(A)
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to – 
1. Develop skills to determine antigen and antibody in the sample by various methods.
2.  Perform blood typing assays and differentiate between blood cells.
Course Outcomes: 

Course

Learning outcomes

(at course level)

Learning and 

teaching 

strategies

Assessment

Strategies

Course 

Code

Course Title

24DZOL502(A)

IMMUNOLOGY

PRACTICAL

CO55: Investigate the role of  ELISA in antigen or antibody determination.

CO56: Describe the importance of Ouchterlony double diffusion in qualitative determination of antigens

CO57: Analyze the importance of agglutination reaction in blood groups determination and prepare blood smear to analyse different blood cells CO58: Differentiate between histology of primary and secondary lymphoid organs

CO59: Demonstrate skills and recond the concepts in the form of practical file and reflect on personal learning experiences and experimental outcomes during viva voce.

CO60:Contribute effectively in course-specific interaction

The approach in  teaching:

Interactive 

Lectures, 

Discussion, 

Tutorials, Reading  assignments, 

Demonstration, 

Team teaching

Learning activities  for the students: Self-learning 

assignments, 

Effective questions,  Simulation, 

Seminar 

presentation, 

Giving tasks, Chart  preparation

Assessment 

Strategies

Class test,  Semester end  examinations, 

Quiz, Solving  problems in  tutorials, 

Assignments, 

Presentation.

 

CONTENTS: 
1. Demonstration of lymphoid organs. 
2. Histological study of spleen, thymus and lymph nodes through slides/ photographs 
3. Preparation of stained blood film to study various types of blood cells.
4. Ouchterlony’s double immuno-diffusion method. 
5. ABO blood group determination. 
6. Demonstration of ELISA 
 
 
SCHEME FOR PRACTICAL EXAMINATION:

Scheme for CA End Practical Examination

Exercise  1

Exercise II

File

Viva

Attendance

Total

 

5

 

 

5

 

10

 

5

 

5

 

30

Scheme for Semester End Practical Examination

Exercise I

Exercise II

Exercise III

Exercise IV

Spotting

File

Viva

Total

8

8

8

8

6x3=18

10

10

70

 

Essential Readings: 
ESSENTIAL READINGS: 
● Roitt’s essential Immunology, P. Delves, et al., 11th ed., Blackwell Publishing, 2006 ∙ 
● Kindt, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006). Immunology, VI Edition. W.H. Freeman and Company. 
* The experiments can be performed depending upon usage of animals in UG Courses. 
 
SUGGESTED READINGS: 
● Kindt, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006). Immunology, VI Edition.  W.H. Freeman and Company. 
● David, M., Jonathan, B., David, R. B. and Ivan R. (2006). Immunology, VII Edition, Mosby, Elsevier Publication. 
● Abbas, K. Abul and Lechtman H. Andrew (2003.) Cellular and Molecular Immunology. V Edition. Saunders Publication.
 
e-CONTENT: 
● Immunology- ePG pathshala- Inflibnet centre- 
 
e-JOURNAL: 
● Immunology- Wiley online Library- ISSN 1365-2567 
● Scandinavian Journal of Immunology-Online ISSN: 1365-3083
 
Academic Year: 
2024-2025
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