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It is a University which is committed to excellence and overall development of the student and one where talent is nurtured and honed in the best possible manner. Come, be a part of our never-ending journey of accomplishments and accolades.

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Admissions

It is a University which is committed to excellence and overall development of the student and one where talent is nurtured and honed in the best possible manner. Come, be a part of our never-ending journey of accomplishments and accolades.

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Placements

Excellent education not only imparts knowledge, but also paves the way for a promising and successful career. The Placement Cell at The IIS University has numerous success stories to narrate in this regard...

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Life@IIS Univ

Life @ IISU is nothing less than a kaleidoscope of myriad hues; complete with vibrant events and memorable occasions to cherish for a lifetime.

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Academics

Taking the age-old tradition of enlightenment through education forward, The IIS University - in its continuous pursuit of excellence - firmly believes that an institution’s academic profile determines its worth and value. Therefore, on offer are a wide variety of both conventional and contemporary programmes of study.

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Research

To stimulate curiosity and cater to the global needs of a dynamic education, The IIS University - since inception - has been promoting and encouraging research-based experiential learning among all its students.

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About Us

The IIS University, with its perfect amalgamation of innovation, inspiration and synergy, aims to be a model institution for students across disciplines and programmes.

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IIS Univ
  • Admissions

    It is a University which is committed to excellence and overall development of the student...

  • Placements

    Excellent education not only imparts knowledge, but also paves the way for...

  • Life@IIS Univ

    Life @ IISU is nothing less than a kaleidoscope of myriad hues; complete with...

  • Academics

    Taking the age-old tradition of enlightenment through education forward,...

  • Research

    To stimulate curiosity and cater to the global needs of a dynamic education,...

  • About Us

    The IIS University, with its perfect amalgamation of innovation, inspiration...

IIS (Deemed to be University)

Gurukul Marg, SFS, Mansarovar, Jaipur 302020, (Raj.) India

Phone:- +91-141-2400160-61, 2397906-07,

Fax: 2395494,2781158, Toll Free No.: 1800 180 7750

Tools and Techniques | Zoology

Tools and Techniques

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Paper Code: 
ZOL-222
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course objectives:
This course will enable the students to -
1. Synthesize basic understanding of diverse techniques from microscopy to spectroscopy, colorimetry, chromatography, electrophoresis, ELISA, tissue culture to cloning etc.
2. Highlight the modern tools, techniques and methodologies. 
 
 

Course

Learning outcomes

      (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL222

Tools

&

Techniques

 

The students will  –

CO37: Develop an understanding of different techniques, its principle of working and its correct application.

CO38: Appraise the detailed knowledge of maintenance of laboratory equipment/ tools, safety hazards and precautions.

CO39:  Analyse the technique of cell and tissue culture and an idea on various reproductive technologies

CO40: Develop an understanding of the principle and applications of radioisotopes in biological sciences and detection and measurement of radiation.

CO41: Intercept the knowledge about modern tools, techniques and methodologies.

CO42: Evaluate practical and theoretical aspects of different instruments.

 

Approach in teaching:

Interactive Lectures, Discussion, PowerPoint presentations, Assignments, Demonstration, Guest lectures

Learning activities for the students:

Self learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

Class test

Semester end examinations

Quiz

Assignments

Presentation

Individual and group presentations

 

15.00
Unit I: 
UNIT I
Principle and application of:-
● Light microscopy
● Phase contrast microscopy
● Polarized microscopy
● Fluorescence microscopy
● Transmission electron microscopy
● Scanning electron microscopy
● Confocal scanning microscopy
 
15.00
Unit II: 
UNIT II

Principle and application of:-

  • Ultracentrifugation :Differential and density gradient
  • Electrophoresis: Agarose, PAGE, Isoelectric- focussing points and Capillary electrophoresis
  • Chromatography: Paper, TLC, GLC, HPLC,Ion- exchange and Affinity chromatography
  • Lamberts – Beer’s Law and Calorimetry,
  • Spectrophotometer: Fluorescence, UV, NMR,ESR.

 

10.00
Unit III: 
UNIT III
 
● Principle and application of radiation techniques in biology
• Radioisotopes and half life of isotopes
• Tracer techniques in biology
• Autoradiography
• Geiger Muller counter
• Scintillation Counter
● Freeze techniques: Freeze –drying, Freeze substitution, Freeze fracture and freeze itch
● X-ray diffraction
● Flow cytometry/fluorescence activated cell sorter
 
10.00
Unit IV: 
UNIT IV
Principles and technique of:-
● Nucleic acid hybridization and cot curves
● Sequencing of nucleic acids
● Blotting techniques(Southern, Northern andWestern)
● Polymerase chain reaction
● Screening of genomic and complementary DNA libraries
 
 
10.00
Unit V: 
UNIT V
● Hybridoma technology
● Embryosexing
● Assisted reproductive technologies
● Cell culture techniques:-culture media, primary and secondary culture, cell line, transformation techniques (in brief) Cell proliferation measurements, Cell viability test
 
Essential Readings: 
BOOKS RECOMMENDED:
 
● Cell and Molecular Biology: Concepts and Experiments, (4th edition), G Karp, John Wiley & sons, Inc., 2005.
● Principles and Techniques of Biochemistry and Molecular Biology ,(6th edition), K Wilson and J Walker (editor), Cambridge University Press, 2007
● Introduction to Instrumental analysis; Robert Braun:, Mc –Graw hill,
● Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc, 1987
● Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000
● Fundamental Laboratory Approaches for Biochemistry and Biotechnology, A J Ninfa., D P Ballou, Fitzgerald science press, Inc., 1998.
● Principles and Practice of Bioanalysis, R F Venn, Taylor and Francis, 2003
● Bioinstrumentation, J G Webster, John Wiley & Sons Inc. 2004
● Protein Purification Principles and Practice, (3rd edition), R K Scopes, Spring International, 2004
● Spectroscopy for the Biological Sciences, G GHames, John Wiley & Sons Inc. 2005
 
Academic Year: 
2021-2022
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