PRACTICAL

Paper Code: 
ZOL125
Credits: 
9
Contact Hours: 
270.00
Max. Marks: 
100.00
Objective: 

Course objectives:

This course will enable the students to –

  1. Design and conduct experiments, using a broad variety of experimental techniques, and interpret the data obtained to draw conclusions about whether the results do or do not support a hypothesis being tested and whether the experimental design was appropriate.
  2. Work collaboratively with other students and with faculty, understanding that most scientific knowledge/conclusions draw(s) on multiple sources of data and multiple experimental approaches.
  3. Learn “best approaches” for conducting experiments including laboratory safety, data replication and quality control, record keeping, and other aspects of “responsible conduct of research”.
  4. Gain familiarity with an area of science such that the students can present their work to a professional audience and also discuss their work with a general audience.

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL 125

Practical

CO16: Identify and describe different laboratory specimens based on their external features and appearance and their categorical relation with various phylum.

CO17: Students will get hands-on experience of various fixation and staining techniques using different biological specimens.

CO18: Enhance their knowledge about different mitotic stages by preparing temporary slides of the onion root tip and visualizing the stages by their key features.

CO19: Learn various qualitative and quantitative biochemical techniques to differentiate between different biomolecules based on their physical and chemical properties.

CO20: Students will be able to analyse the effect of temperature on the enzymatic activity and gain hands on expertise in separation of various solutes using chromatographic techniques.

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students:

Self learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

 

 

Class test

Semester end examinations

Viva voce

Assignments

Presentation

Individual and group projects

 

Invertebrates

Identification, classification & study of distinguishing features of representatives from various groups.
 

  •     Phylum Protozoa- Polystomella, Formanifera, Opalina, Paramecium(Fission & conjugation) Vorticella, Euglena, Trypanosoma,Monocystis,Plasmodium.
  •     Phylum Porifera-Sycon L.S & T.S, Spicules, Spongin fibres, Leucosolenia, Euplectella
  •     Phylum Cnidaria- Obelia,( polyp & Medusa), Millepora, Physalia, Pennatula, Metridium, Madrepora, Alcyonium, Gorgonia, Aurelia.
  •     Phylum Helminthes- Ascaris, Taenia, Planaria
  •     Phylum Annelida- Pontobdella, Aphrodite, Leech, Polygordius, Chaetopterus, Nereis, Heteronereis, Arenicola.
  •     Phylum Arthropoda- Cyclops, Peripatus, Balanus, Lepas, Hippa, Belostoma, Limulus, Eupagurus, Julus, Scolopendra, Praying mantis.
  •     Phylum Mollusca- Murex, Bulla, Cardium, Arca, Turritella, Pinctada, Cypraea, Octopus, Nautilus.
  •     Phylum Echinodermata- Echinus, Holothuria, Antedon, Asterias
  •     Minor Phyla- Bugula, Plumatella, Crestatella, Pectinella,Phoronis, Dendrostoma

    Larval Stages: Planula, Redia, Miracidium, Sporocyst,Cercaria,Metacercaria Trochophore, Nauplius, Zoea, Mysis, Phyllosoma, Trilobite larva of Limulus, Glochidium, Bipinnaria, Echinopluteus, Auricularia, Tornaria

Dissections :
 

  •  Cockroach: General anatomy, Reproductive system, Nervous system
  • Mouthparts:Cockroach & Mosquito

Biosystematics, taxonomy and evolution
 

  •     Composition and assessment of the taxonomic diversity/biodiversity in a habitat (e.g. grassland, arid land, wet land etc.)
  •     Estimation of gene and genotypic frequencies in light of Hardy-Weinberg law based on facial traits/thumb prints, ABO blood group data etc.
  •     Project: Preparation of models showing the status of certain taxa or species in a particular habitat.
  •     Identification of Phytoplanktons and Zooplanktons from fresh water samples.

Cell Biology
 

  •     Preparation of different cell types: Liver Hepatocytes , Adipocytes, Spleen cells
  •     Study of Polytene chromosomes in salivary glands of Chironomus larva/Drosophila
  •     Light microscopic examination and preparation of tissue sections.
  •     Study of mitotic and meiotic chromosomes from permanent prepared slides.
  •     Preparation of slides for mitotic study.
  •     Preparation of slides for meiotic study in the testis of grasshopper.
  •     Preparation of multipolar nerve cells from the spinal cord of a mammal.
  •     Barr body preparation in buccal epithelial cells.

Biochemistry
 

  •     Qualitative analysis of Lipid, protein and Carbohydrate samples
  •     Colorimetric/Spectrophotometric estimation of :
  •     Carbohydrate- glycogen,
  •     cholesterol
  •     Protein
  •     Nucleic acids: DNA,RNA
  •     Study of temperature effect on enzyme action: salivary amylase, liver catalase
Essential Readings: 

Suggested Reading

    Jordon, E.L., Verma P.S. Invertebrate Zoology, S Chand and Co.

    Laboratory manual in Invertebrate & Experimental Zoology, Jacob VyasRamesh Book Depot, New Delhi.

    Practical Zoology, Jacob, D., Bhatia,A.L., Kohli,K.S., Vyas,D.K.Ramesh Book Depot, New Delhi.

    Biology of Animals. C.P. Hickmann,  L.S. Roberts and A. Larson. McGraw Hill Company, New York.

    Practical Biochemistry:Principles and Techniques; Keith Wilson& John Walker;CambridgeUniversity Press.

    Introductory Practical Biochemistry by S.K Sawhney and Randhir Singh Alpha Science

    A Primer of Population Genetics. Hart, D.L. Suinuaer Associates, Inc, Massachusetts.

Academic Year: 
2020-2021
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