Practical

Paper Code: 
ZOL 215
Credits: 
4
Contact Hours: 
120.00
Max. Marks: 
100.00
Objective: 

Course objectives:

This course will enable the students to -

  1. Study of invertebrate laboratory specimens based on their external features and appearance
  2. To gain knowledge about various molecular biology techniques including gel electrophoresis and chromatography and their applications in the research field.
  3. Study of wild and mutant strains of Drosophila.
  4. Performance of qualitative and quantitative biochemical techniques to differentiate between different biomolecules based on their physical and chemical properties.
  5. Analysis of different modes of central tendency, standard deviation and variance.

Course Outcomes (COs):

 

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

ZOL-215

Practical

 

The students will be able to

CO 41: Identify and describe different laboratory specimens based on their external features and appearance and their categorical relation with various phylum.

CO 42: Get hands-on experience of various molecular biology techniques including gel electrophoresis and chromatography and would learn about their applications in the research field.

CO 43: Enhance their knowledge about wild and mutant strains of Drosophila and their important structures.

CO 44: Learn various qualitative and quantitative biochemical techniques to differentiate between different biomolecules based on their physical and chemical properties.

CO 45: Perform calculations of different modes of central tendency, standard deviation and will be able to calculate variance.

The approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical, demonstration by virtual software.

 

Class test

Semester end examinations,

Quiz,

Assignments,

Presentation

Surprise test

Open book test

Biochemistry II

Colorimetric/Spectrophotometric estimation of :

    Carbohydrate-Glucose
    Preparation of Phosphate buffer
    Isolation of protein (casein) from milk
    Study of temperature effect on enzyme action: Salivary amylase

Animal Diversity III

Study of museum specimens and slides:

Phylum  Annelida-Nereis, Heteronereis-parapodia, Earthworm, Leech

Dissection (Demonstration): Earthworm- Anatomy, Digestive, Nervous System

Permanent mount:Septalnephridia, Ovary,Nerve ring.
       
Phylum Arthropoda: Daphnia, Cyclops, Scorpion, King Crab, Crab, Eupagurus, Balanus, Millipede, Termite, Centipede, Praying-mantis, Peripatus

Dissection: (Demonstration): Prawn-General anatomy, Appendages, Nervous & Digestive system

Permanent mount: Hastate plate, Statocyst

Phylum-Mollusca:Chiton, Unio, Dentalium, Nautilus, Loligo, Octopus, Sepia

Phylum Echinodermata: Echinus, Starfish, Cucumaria, Antedon

Larval forms- Miracidium, Sporocyst, Redia, Cercaria,Nauplius,Zoea,Mysis,cypris,Megalopa, Glochidium larva.

Molecular Biology:

    DNA isolation and separation by Agarose gel method
    Vertical and horizontal electrophoresis unit (demonstration only)
    Paper chromatographic separation of amino acids

Genetics &Biostatistics

    Study of Drosophila life cycle & idea about its culture
    Identification of Male & Female Drosophila
    Identification of Wild & mutant (Yellow body, Ebony, Vestigial wings, White eye, Apterus) of  Drosophila
    Study of permanent prepared slide - Sex comb, Salivary gland chromosomes
    Identification of Blood groups (A,B, O & Rh factor)
    Preparation of frequency tables and graphs manually and with help of computers.
    Calculation of Mean, Median, Mode.
    Calculation of standard deviation, variance and standard error of the mean.
    Chi square test for independence of attributes, t test

 

Essential Readings: 

 

  • A Biologist's Guide to Principles and Techniques of Practical Biochemistry, K.Wilson& KH Goulding, ELBS Edition 1986.
  • Wilson,K. and Walker, J. Principles and techniques of practical biochemistry, Cambridge press.
  • Zubay G. Biochemistry, William C. Brown Pub.
  • Standard methods of biochemical analysis.Thimmaiah, S.R. KalyaniPublishers,Noida.
  • Pechenik, J.A. Biology of invertebrates,TataMc-Graw Hill Ltd, New Delhi.
  • Jordon, E.L., Verma P.S. Invertebrate Zoology, S Chand and Co.
  • Practical Zoology, Invertebrates, Lal, S.S. Rastogi Publications, Meerut
  • Practical Zoology, Invertebrates ,Kohli,K.S., Ansari, A.S. Ramesh Book Depot, New Delhi.
  • Laboratory manual in Invertebrate & Experimental Zoology, Jacob Vyas Ramesh Book Depot, New Delhi.
  • Practical Zoology, Jacob, D., Bhatia,A.L., Kohli,K.S., Vyas,D.K. Ramesh Book Depot, New Delhi.
  • Invertebrate Zoology Barnes, RD. W.B.Saunders Co., Philadelphia
  • Biostatistics; Gupta, S.P.;Sultan Chand & Co.
  • Practical Exercises in Cytology, Genetics, Plant Breeding and Biostatistics. Raghuvanshi.

 

Academic Year: 
2020-2021
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