PRACTICAL

Paper Code:

ZOL-503

Credits:

2

Max. Marks:

100.00

Objective:

OBJECTIVES AND OUTCOMES

Design and conduct experiments, using a broad variety of experimental techniques, and interpret the data obtained to draw conclusions about whether the results do or do not support a hypothesis being tested and whether the experimental design was appropriate.
Work collaboratively with other students and with faculty, understanding that most scientific knowledge/conclusions draw(s) on multiple sources of data and multiple experimental approaches.
Learn “best approaches” for conducting experiments including laboratory safety, data replication and quality control, record keeping, and other aspects of “responsible conduct of research”.
Gain familiarity with an area of science such that the students can present their work to a professional audience and also discuss their work with a general audience.
Develop problem solving skills and analytical thinking skills.

Biotechnology and Microbiology:

Principle and working of equipments used in biotechnology laboratory(SDS-PAGE apparatus, horizontal DNA Electrophoresis chamber, Laminar Airflow, Autoclave, Incubator, pH meter, Homogenizer, Ascending and Descending Chromatographic chamber, Thin Layer Chromatographic Apparatus, Microtome)
Preparation and use culture media for microbes
Study of microbes in food material(curd, milk, rotten fruits and vegetables)
Educational tour to any Microbiology lab (Food processing factory, Distillery, Dairy etc.)Candidates will have to submit a report on it.
Culturing of Bacteria from soil/water samples.

Immunology:

Experiments on Antigen and Antibody reaction.

Blood typing
ELISA
Ouchterlony double diffusion

Essential Readings:

Suggested Reading:

Practical Biochemistry: Principles &Techniques; Keith Wilson& John Walker; Cambridge University Press.
Microbiology, A laboratory manual by Cappucino, James,G.
Lab Manual of microbiology & biotechnology by K. R. Aneja. Scientific international private limited.

Academic Year: