PRACTICAL

Paper Code:

24ZOL 225

Credits:

Contact Hours:

270.00

Max. Marks:

100.00

Objective:

Course objectives:

This course will enable the students to –

1. Design and conduct experiments, using a broad variety of experimental techniques, and interpret the data obtained to draw conclusions about whether the results do or do not support a hypothesis being tested and whether the experimental design was appropriate.

2. Work collaboratively with other students and with faculty, understanding that most scientific knowledge/conclusions draw(s) on multiple sources of data and multiple experimental approaches.

3. Learn “best approaches” for conducting experiments including laboratory safety, data replication and quality control, record keeping, and other aspects of “responsible conduct of research”.

4. Develop problem solving skills and analytical thinking skills.

Course Outcomes:

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Cour se Code

Course Title

24ZOL

225

Practical

CO61. Evaluate practical and

theoretical aspects of different

hematological parameters

CO62. Appraise hands-on practice of

various microscopic techniques,

occulometry, centrifugation and UV-

visible spectroscopy.

CO63. Appraise hands-on experience

of various molecular biology

techniques, chromosomal aberration

and would learn about their applications in the research field.

CO64. Evaluate various qualitative

and quantitative parameters of soil

and water

CO65. Reflect on personal learning

experiences and experimental

outcomes during viva voce

assessments.

CO66. Contribute effectively in

course-specific interaction

Approach in

teaching:

Interactive

Lectures,

Discussion,

Tutorials,

Demonstration,

Team teaching

Learning

activities for the

students:

Self learning

assignments,

Effective

questions,

Simulation,

Giving tasks,

Field

practical

Class test

Semester

end

examination

s Viva voce

Presentation

Unit I:

UNIT I

CONTENTS

General Physiology

● Comparison of RBC and WBC in different groups of mammals.

● Photometric determination of hemoglobin in blood sample.

● Determination of MCV, MCH,MCHC of the given sample of blood

● Demonstration of the following in blood:Clotting time, Erythrocyte sedimentation rate, Haemolysis and Crenation

● Determination of Haematocrit value of blood sample.

Tools and Techniques

● Microscopes

● Standardization of Occulometer and measurements of tubular diameter, cell heights.

● Measurement of diameter of thyroid follicles.

● To study the structure & function of Centrifuge.

● To study the principle of UV & visible light spectroscopy.

● To study the principle of cyclomixer.

● Flow cytometry

● To observe the subcellular fractionation of rat liver.

● Alizarin preparation.

Molecular Biology & Biotechnology

● Chromosomal counting in bone marrow cells of a mammal

● Isolation of DNA

● Restriction digestion of DNA

● Agarose gel electrophoresis of DNA

● Separation of amino acids on Course and Thin layer Chromatography

● Separation of protein on SDS PAGE

Environmental Science and Wildlife Management

Water Evaluation Analysis (minimum of five analysis methods)

● Determination of chloride in the given water sample.

● Estimation of total hardness of given water sample.

● To determine the acidity of water.

● To determine the alkalinity of water.

● To estimate total solid in water.

● To estimate the total dissolved solid in the water sample.

● To determine the dissolved oxygen in a given water sample.

● To determine the BOD of a given water sample.

● To determine the free CO2 of a given water sample.

● To estimate the Calcium hardness of water.

● To estimate Magnesium hardness of a given water sample.

Soil Analysis (minimum of five analysis methods)

● Determination of organic carbon and organic matter in soil sample.

● Determination of CO3-& HCO3-in soil sample.

● Estimation of chlorophyll in plant material.

● Determination of leaf area index and percent leaf injury of sample from polluted sites.

● Determination of residual chloride in water sample.

● Determination of moisture in soil.

● Determination of frequency, density and abundance of community of quadrant

● Determination of dust holding capacity of leaves.

● Determination of bulk density of the given soil sample.

● Determination of water holding capacity of soil.

● Test for presence of inorganic salts in soil.

A visit to a sanctuary/National Park./Educational Tour (Preparation of Report)

Essential Readings:

● Principles and Techniques in Biochemistry and Molecular Biology, Edited by Keith Wilson and John Walker, Cambridge University Press, (6th Edition),2007.

● Handbook of methods in Environmental Studies,Vol 2 (Air, noise, soil and overburden analysis) Maiti,S.K., Jaipur Publications.

● Handbook of Methods in Environmental Studies, Vol I, Water and Wastewater, Maiti,S.K., Jaipur Publications.

● Fundamental Laboratory Approaches for Biochemistry and Biotechnology, A J Ninfa., D P Ballou, Fitzgerald science press, Inc.,1998.

References:

SUGGESTED READING:

● Sembulingam, K.Sembulingam. Essentials of medical Physiology, Jaypee Brothers Medical Publishers, New Delhi.

● TextBook of Medical Physiology; Chatterjee, M.N and Shinde, R.; Jaypee brothers.( 2007)

● Principles of Wildlife management; James, A. Bailey; John Wiley & sons

● Introduction to Instrumental analysis; Robert Braun: Mc –Graw hill, International eds.

● Environmental Science, Santra S.C., New Central Book Agency Pvt Ltd., Kolkata

● Environmental Science, Cunningham andSaigo

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