Coming soon
Practical
Course objectives:
This course will enable the students to –
- Design and conduct experiments, using a broad variety of experimental techniques, and interpret the data obtained to draw conclusions about whether the results do or do not support a hypothesis being tested and whether the experimental design was appropriate.
- Work collaboratively with other students and with faculty, understanding that most scientific knowledge/conclusions draw(s) on multiple sources of data and multiple experimental approaches.
- Learn “best approaches” for conducting experiments including laboratory safety, data replication and quality control, record keeping, and other aspects of “responsible conduct of research”.
- Gain familiarity with an area of science such that the students can present their work to a professional audience and also discuss their work with a general audience.
Course Outcomes (COs):
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Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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ZOL 125 |
Practical |
The students will –
CO22: Illustrate different laboratory specimens based on their external features and appearance and their categorical relation with various phylum. CO23: Students will get hands-on experience of various fixation and staining techniques using different biological specimens. CO24: Enhance their knowledge about different mitotic stages by preparing temporary slides of the onion root tip and visualizing the stages by their key features. CO25: Experiment various qualitative and quantitative biochemical techniques to differentiate between different biomolecules based on their physical and chemical properties. CO26: Analyse the effect of temperature on the enzymatic activity and gain hands-on expertise in separation of various solutes using chromatographic techniques. |
Approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching
Learning activities for the students: Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical
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Class test Semester end examinations Viva voce Assignments Presentation Individual and group projects |
Invertebrates
Identification, classification & study of distinguishing features of representatives from various groups.
- Phylum Protozoa- Polystomella, Formanifera, Opalina, Paramecium(Fission & conjugation) Vorticella, Euglena,Trypanosoma,Monocystis,Plasmodium.
- Phylum Porifera-Sycon L.S & T.S, Spicules, Spongin fibres, Leucosolenia, Euplectella
- Phylum Cnidaria- Obelia,(Polyp & Medusa), Millepora, Physalia,Pennatula, Metridium, Madrepora, Alcyonium, Gorgonia, Aurelia.
- Phylum Helminthes- Ascaris, Taenia,Planaria
- Phylum Annelida- Pontobdella, Aphrodite, Leech, Polygordius,Chaetopterus, Nereis, Heteronereis,Arenicola.
- Phylum Arthropoda- Cyclops, Peripatus, Balanus, Lepas, Hippa, Belostoma, Limulus, Eupagurus, Julus, Scolopendra, Prayingmantis.
- Phylum Mollusca- Murex, Bulla, Cardium, Arca, Turritella, Pinctada,Cypraea, Octopus,Nautilus.
- Phylum Echinodermata- Echinus, Holothuria, Antedon, Asterias
- Minor Phyla- Bugula, Plumatella, Crestatella, Pectinella,Phoronis,Dendrostoma
- Larval Stages: Planula, Redia, Miracidium, Sporocyst,Cercaria,Metacercaria, Trochophore, Nauplius, Zoea, Mysis, Phyllosoma, Trilobite larva ofLimulus, Glochidium, Bipinnaria, Echinopluteus, Auricularia, Tornaria
Dissections:
- Cockroach: General anatomy, Reproductive system, Nervous system Mouthparts:Cockroach and Mosquito
Biosystematics, taxonomy and evolution
- Composition and assessment of the taxonomic diversity/biodiversity in a habitat (e.g. grassland, arid land, wet land etc.)
- Estimation of gene and genotypic frequencies in light of Hardy-Weinberg law based on facial traits/thumb prints, ABO blood group data etc.
- Project: Preparation of models showing the status of certain taxa or species in a particular habitat.
- Identification of Phytoplanktons and Zooplanktons from fresh water samples.
Cell Biology
- Preparation of different cell types: Liver Hepatocytes , Adipocytes, Spleen cells
- Study of Polytene chromosomes in salivary glands ofChironomus larva/Drosophila
- Light microscopic examination and preparation of tissue sections.
- Study of mitotic and meiotic chromosomes from permanent prepared slides.
- Preparation of slides for mitotic study.
- Preparation of slides for meiotic study in the testis of grasshopper.
- Preparation of multipolar nerve cells from the spinal cord of mammal.
- Barr body preparation in buccal epithelial cells.
Biochemistry
- Qualitative analysis of Lipid, protein and Carbohydrate samples
- Colorimetric/Spectrophotometric estimation of:
- Carbohydrate- glycogen,
- cholesterol
- Protein
- Nucleic acids:DNA,RNA
- Study of temperature effect on enzyme action: salivary amylase, liver catalase
