Molecular Biology and Biotechnology

Paper Code: 
ZOL 223
Unit I: 

DNA Replication

  • Prokaryotic & eukaryotic DNA replication
  • Mechanics of DNA replication
  • Enzymes and accessory proteins involved in DNA replication
  • DNA Repair mechanism
Unit II: 


  • Prokaryotic and Eukaryotic  transcription
  • RNA polymerases
  • General and specific transcription factors

Post transcriptional modification in RNA

  • 5’ cap formation
  • Transcription termination
  • 3’ end processing and polyadenylation
  • Splicing
  • Nuclear export of mRNA
  • mRNA stability
Unit III: 


  • Genetic code
  • Prokaryotic and eukaryotic translation
  • Translational machinery
  • Mechanisms of  Initiation, Elongation and termination
  • Regulation of Translation
  • Co and post translational modifications of protein


Homologous recomibation: Holiday junction,

Site FLP/ FRT and Cre/lox recombination

Rec A and other recombinases

Gene targeting, Gene disruption



Unit IV: 

Molecular mapping of Genome

  • Genetic and physical maps
  • Southern and fluorescence in situ hybridization for genome analysis
  • Molecular markers in genome analysis: RFLP,RAPD and AFLP analysis


Unit V: 

Principle and technique of genetic engineering

·         Cutting and joining of DNA molecules

·         Cloning strategies, gene library and cDNA

·         Application of recombinant DNA technology

o   Recombination selection and screening

o   Nucleic acid probes and their application

Transgenic animals and knock outs    

Essential Readings: 
  1. Molecular Biology of the gene; Watson J.D.; Benzamin/ Cummings.
  2. Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994.
  3. Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American Books,USA,1994.
  4. Cell & Molecular Biology, (8th edition), E D P Roberties & E M F Roberties,  Lippincott Williams & Wilkins, 2005
  5. The cell, (5th edition), R C Swanson and P C Webster , Prentice hall of India Pvt. Ltd., 1990
  6. Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc, 1987
  7. Cell and Molecular Biology: Concepts and Experiments, (4th edition), G  Karp, John Wiley & sons, Inc., 2005
  8. The cell. A Molecular Approach, (4th edition), G H Cooper and R E Hausman, ASM Press, 2007
  9. Gene VII, Indian eds. Lewin, B. Oxford university press, Bombay
Academic Year: