Molecular Biology and Biotechnology

Paper Code: 
ZOL-223
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course objectives: 
This course will enable the students to – 
 
1. Develop an understanding of the life processes at the subcellular and molecular levels. 
2. Differentiate and evaluate important aspects of DNA and molecular genetics including gene cloning, sequencing and gene mapping in addition to the powerful techniques that revolutionized the pharmaceutical, health and agricultural industries.
 
 

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL 223

 

Molecular Biology And Biotechnology

The students will –

CO43:Develop an understanding of concepts, mechanisms and evolutionary significance and relevance of molecular biology in the current scenario.

CO44:Differentiate and evaluate in recombinant DNA technology which holds application in biomedical and genomic science, agriculture etc.

CO45: Appraise the fundamental understanding of Molecular Biology which will help in career building in all these fields.

CO46: Apply their knowledge in problem solving and future course of their career development in higher education and research.

CO47: Evaluate practical and theoretical aspects of different molecular techniques.

 

Approach in teaching:

 

Group Discussion, Reading assignments, PowerPoint presentations

Demonstration, Guest lecture

 

Learning activities for the students:

Effective questions, Simulation, Seminar presentation and Self learning assignments, Field practical

 

Class test

Semester end examinations

Quiz

Assignments

Presentation

Model or chart preparation

 

9.00
Unit I: 
UNIT I
DNA Replication
● Prokaryotic & eukaryotic DNA replication
● Mechanics of DNA replication
● Enzymes and accessory proteins involved in DNA replication
● DNA Repair mechanism
 
11.00
Unit II: 
UNIT II
Transcription
● Prokaryotic and Eukaryotic transcription
● RNA polymerases
● General and specific transcription factors
 
Post transcriptional modification in RNA
● 5’ cap formation
● 3’ end processing and polyadenylation
● Splicing
● Nuclear export of mRNA
● mRNA editing
 
12.00
Unit III: 
UNIT III
Translation
● Genetic code
● Prokaryotic and eukaryotic translation
● Translational machinery
● Mechanisms of Initiation, Elongation and termination
● Regulation of Translation
● Co and post translational modifications of protein Recombination
● Homologous recombination: Holiday junction,
● Site FLP/ FRT and Cre/lox recombination
● Rec A and other Recombinases
● Gene targeting, Gene disruption
 
14.00
Unit IV: 
UNIT IV
Molecular mapping of Genome
● Genetic and physical maps
● Southern and fluorescence in situ hybridization for genome analysis
● Molecular markers in genome analysis: RFLP, RAPD and AFLP analysis
 
14.00
Unit V: 
UNIT V
Principle and technique of genetic engineering
● Cutting and joining of DNA molecules
● Cloning strategies, gene library and cDNA
● Application of recombinant DNA technology
❖ Recombination selection and screening
❖ Nucleic acid probes and their application Transgenic animals and knockouts
Essential Readings: 
● Molecular Biology of the gene; Watson J.D.; Benjamin /Cummings.
● Molecular Biology of the Cell (2nd Edition) B. Alberts, D. Bray, J. Lewis, M. Raff, K. Roberts, and J.D. Watson, Garland publishing. Inc., New York, 1994Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific AmericanBooks,USA,1994.
● The cell. A Molecular Approach, (4th edition), G H Cooper and R E Hausman, ASM Press,2007.
● Cell & Molecular Biology, (8th edition), E D P Robertis & E M F Robertis, Lippincott Williams & Wilkins,2005
● The cell, (5th edition), R C Swanson and P C Webster , Prentice hall of India Pvt. Ltd., 1990
● Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc,1987
● Cell and Molecular Biology: Concepts and Experiments, (4th edition), G Karp, John Wiley & sons, Inc.,2005
● Gene VII, Indian eds. Lewin, B. Oxford university press,Bombay
 
Academic Year: 
2021-2022
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