IMMUNOLOGY PRACTICAL

Paper Code: 
24DZOL516A
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course objectives: 
This course will enable the students to – 
1. Develop skills to determine antigen and antibody in the sample by various methods.
2.  Perform blood typing assays and differentiate between blood cells.
 
Course Outcomes: 

Course

Learning outcomes

(at course level)

Learning and 

teaching strategies

Assessment

Strategies

Course Code

Course Title

24DZOL516A

Immunology

Practical

CO:127 Investigate the role of ELISA in antigen or antibody determination.

CO:128 Describe the importance of Ouchterlony double diffusion in qualitative determination of antigens

CO:129 Analyze the importance of agglutination reaction in  blood  groups determination and demonstrate different blood cells by preparing blood smear.

CO:130 Differentiate between histology of primary and secondary lymphoid organs

CO:131 Demonstrate skills and record the concepts in the form of  practical file and reflect on personal learning experiences and experimental outcomes during viva voce

CO:132 Contribute effectively in course-specific interaction

The approach in  teaching:

Interactive Lectures, 

Reading  assignments, Demonstration,  Team teaching

 

Learning 

activities for the students:

Self-learning assignments, 

Effective questions, 

Simulation,

Assessment 

Strategies

Class test, Semester  end examinations, Solving  problems in tutorials,  Assignments, 

Presentation.

 

1. Demonstration of lymphoid organs. 
2. Histological study of spleen, thymus and lymph nodes through slides/ photographs
 3. Preparation of stained blood film to study various types of blood cells. 
4. Ouchterlony‘s double immuno-diffusion method. 
5. ABO blood group determination. 
6. Demonstration of : ELISA 
* The experiments can be performed depending upon usage of animals in UG Courses. 

SCHEME FOR PRACTICAL EXAMINATION:

Scheme for CA  End Practical Examination

Exercise  1

Exercise II

File

Viva

Attendance

Total

 

 

5

 

 

5

 

10

 

5

 

5

 

30

 

Scheme for Semester End Practical Examination

Exercise I

Exercise II

Exercise III

Exercise IV

Spotting

File

Viva

Total

8

8

8

8

6x3=18

10

10

70

 

 

Essential Readings: 
SUGGESTED READINGS 
● Kindt, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006). Immunology, VI  Edition. W.H. Freeman and Company. 
● David, M., Jonathan, B., David, R. B. and Ivan R. (2006). Immunology, VII Edition,  Mosby, Elsevier Publication. 
● Abbas, K. Abul and Lechtman H. Andrew (2003.) Cellular and Molecular Immunology.  V Edition. Saunders Publication.
 
Academic Year: 
2024-2025
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