Cell and Molecular Biology I

Paper Code: 
ZOL 323 B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL 323B

 

Cell And Molecular Biology

Students will –

CO77: Examine the structure and function of biomembrane and its role in active and passive transport.

CO78: Elaborate the role of Cytoskeleton, Cell Junctions and cellular adhesion molecules in intracellular movements and cell to cell and cell to matrix interactions.

CO79: Acquire the detailed knowledge of different pathways related to cell signaling and apoptosis thus enabling them to understand the importance of cell cycle regulation checkpoints.

CO80: Perceive the detailed knowledge about chromosomal organization of genes and noncoding DNA.

CO81: Conclude the role of DNA binding motifs in regulation of gene expression.

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks

 

Class test

Semester end examinations,

Quiz

Assignments,

Presentation

Surprise test

Open book test

 

12.00
Unit I: 
UNIT I
Molecular structure of genes and chromosomes
● Molecular definition of gene.
● Chromosomal organization of genes and non-coding DNA.
● Mobile DNA.
● Functional rearrangement of chromosomal DNA
● Mutation: Types, causes and detection, mutant types – lethal, conditional, biochemical, loss of function, gain of function, germinal vs somatic mutants, insertional mutagenesis.
● Structural and numerical alterations of chromosomes: Deletion, duplication, inversion, translocation, ploidy and their genetic implications
 
12.00
Unit II: 
UNIT II

Regulation of Gene Expression

  • Operon Concept.
  • Catabolite Repression.
  • Positive and Negative regulation.
  • Inducers and co-repressors.
  • Attenuation mechanism

DNA binding & Protein dimerization domains.

  • Homeodomain proteins.
  • Zinc finger proteins.
  • Leucine-Zipper proteins.
  • Helix-loop-helix proteins   
12.00
Unit III: 
UNIT III

Cell cycle

  • Eukaryotic cell cycle-Gl, S, G2 and M Phases.
  • Cell cycle and checkpoints.
  • Molecular basis of cell cycle regulation.
  • Cyclins and cyclin-dependent kinases.
  • Regulation of CDK-cyclin activity

Cell Death: Apoptosis and necrosis

  • Extrinsic pathway
  • Intrinsic pathway
  • apoptotic and antiapoptotic genes
12.00
Unit IV: 
UNIT IV

Cancer

  • Properties of cancer cell and onset of cancer
  • Causes of cancer
  • Retrovirus
  • Mechanism of conversion of Proto-oncogenes to oncogenes
  • Tumor suppressor genes

​       Aging: The biology of senescence:

  • Maximum life span and life expectancy
  • causes of aging

 

 

12.00
Unit V: 
UNIT V
 
  • Mendelian principles: Dominance, segregation, independent assortment
  • Interaction of genes: Codominance, incomplete dominance, dominant and recessive epistasis, complementary genes, supplementary genes, duplicate genes, lethal genes
  • pleiotropy, penetrance and expressivity, linkage and crossing over, sex linkage, sex limited and sex influenced characters.
  • Pedigree analysis:
  • autosomal recessive traits-
  • autosomal dominant traits:
  • X linked recessive traits
  • X linked dominant traits
  • Y linked traits
Essential Readings: 
● IWASA, J., MARSHALL, W. F., & KARP, G. (2016). Karp's cell and molecular biology: concepts and experiments.
● COOPER, G. M., & HAUSMAN, R. E. (2009). The cell: a molecular approach. Washington, D.C., ASM Press.
● Nelson et al, Lehninger’s Principles of Biochemistry, 4th Edition, McMillan, 2006.
● Alberts et al, Molecular Biology of The Cell, 2nd Edition , Garland 2007.
● Lewin, Genes IX, 9th Edition , Jones & Bartlett, 2007
● .Molecular Biology of the Cell ; Lewis and Watson
● Genes VII : Benjamin Lewin
● Lewis, Human Genetics, 7th Edition, WCB & McGraw, 2007
SUGGESTED READINGS:
● Pastemak, An Introduction to Molecular Human Genetics, 2nd Edition, Fritzgarald, 2005.
● Strachen and Read, Human Molecular Genetics, 3rd Edition, Garland Sci. Publishing, 2004.
● Structural Chemistry and Molecular Biology Alexander Rich and Norman Davidson
● The Chemical Foundations of Molecular :James D. Watson
● Human Cytogenetic Techniques and Clinical Applications HemaPurandare and Arnit Chakravarty
● Progress in Nucleic Acid Research and Molecular Biology Vol. 9 J. N. Davidson
● Essentials of Human Genetics Bhatnagar,Kothari,Mehta
● Principles and Practice of Medical Genetics, by Emery, A.E.H and D.L. Rimoin (Eds_ (1990-2nd edition) Churchill Livingstone, Edinburgh.
● Molecular Basis of Inherited Diseases, (6th Edition-1989) by Scriver, C.R. A.L. Beudit, W.S. Sty ab nd D. Valle (Eds0 McGrawHill, New York.
● Human Genetics by S.D. Gangane (2nd edition-Reprint 2001), B.L Churchill Livingstone Pvt. Ltd., New Delhi.
● Genetics in Medicine by M.W. Thompson et al, 5th Edition, W.B. Sounders Company, London
● Genetic basis of common diseases by R. A. King et al, Oxford University Press.
● Mendelian inheritance in Man by Mc. Kusick V.A. (1998), 12th Edition, John Hopsins University Press, Baltimore.
 
e- RESOURCES:
● Cell biology -NPTEL- https://nptel.ac.in/courses/102103012
● Molecular Biology-NPTEL-https://nptel.ac.in/courses/102104052
e- JOURNAL: 
● Molecular and Cellular Biology
● BMC Cell and Molecular Biology- ISSN: 2661-8850

 

Academic Year: 
2023-2024
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