Biotechnology & its Applications

Paper Code: 
ZOL 511
Contact Hours: 
Max. Marks: 

Basic concepts in Genetic Engineering

  • Enzymology of Genetic engineering- Restriction enzymes, DNA ligase

Cloning vehicles-plasmids, cosmids, lambda phage & yeast plasmids


Introduction of cloned genes into the host cells

  • Transformation
  • Transduction
  • Transgenic animals, their uses and biotechnology

Brief account of cloning


Analysis & expression of cloned gene in host cell, Southern blotting, Northern blotting, Polymerase chain reaction, DNA probes

Genomic library & cDNA library


Hybridoma technology, Monoclonal antibodies and their applications

Genomic research-its advantages and disadvantages

 Elementary idea of Human Genome Project


Biotechnology in Medicine (outline idea only):

  • Antibiotics
  • Vaccines
  • Enzymes
  • Vitamins.
  • Steroids.
  • Artificial Blood.

Potential hazards and regulations of genetic engineering

Essential Readings: 
  • Elementary biotechnology, Gupta, P.K; Rastogi Puplications; Meerut.
  • Immunology; Kuby; W.H; Freeman Publications.
  • R.C. Dubey, A Text Book of Biotechnology, S. Chand and Company, New Delhi.
  • H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd. New Delhi.
  • Molecular Biotechnology. Glick B.R. & Pasternak J. J; ASM Press.
  • Principles of gene manipulation; An introduction to genetic engineering; Old, R.W & Primrose, S.B; Blackwell Science.
  • Immunology; Kuby; W.H; Freeman Publications.
  • D.M. Wear, Immunology ELBS (English) Language Book Society Low Priced edition, Longman Singapore Publishers Ltd.
  • Iyan M. Roitt essential Immunology EIBS Low Priced Edition Black well Scientific Publications.
Academic Year: