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BIOTECHNOLOGY & ITS APLICATIONS
Paper Code:
ZOL-511
Credits:
3
Contact Hours:
45.00
Max. Marks:
100.00
10.00
Unit I:
UNIT I
Basic concepts in Genetic Engineering
Enzymology of Genetic engineering- Restriction enzymes, DNA ligase
Cloning vehicles-plasmids, cosmids, lambda phage & yeast plasmids
9.00
Unit II:
UNIT II
Introduction of cloned genes into the host cells
Transformation
Transduction
Transgenic animals, their uses and biotechnology
Brief account of cloning
5.00
Unit III:
UNIT III
Analysis & expression of cloned gene in host cell, Southern blotting, Northern blotting, Polymerase chain reaction, DNA probes
Genomic library & cDNA library
11.00
Unit IV:
UNIT IV
Hybridoma technology, Monoclonal antibodies and their applications
Genomic research-its advantages and disadvantages
Elementary idea of Human Genome Project
10.00
Unit V:
UNIT V
Biotechnology in Medicine (outline idea only):
Antibiotics
Vaccines
Enzymes
Vitamins.
Steroids.
Artificial Blood.
Potential hazards and regulations of genetic engineering
Essential Readings:
Molecular Biotechnology. Glick B.R. & Pasternak J. J; ASM Press.
Principles of gene manipulation; An introduction to genetic engineering; Old, R.W & Primrose, S.B; Blackwell Science.
Elementary biotechnology, Gupta, P.K; Rastogi Puplications; Meerut.
Immunology; Kuby; W.H; Freeman Publications.
R.C. Dubey, A Text Book of Biotechnology, S. Chand and Company, New Delhi.
H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd. New Delhi.
D.M. Wear, Immunology ELBS (English) Language Book Society Low Priced edition, Longman Singapore Publishers Ltd.
Iyan M. Roitt essential Immunology EIBS Low Priced Edition Black well Scientific Publications.
Academic Year:
