Coming soon
Biological Tools and Techniques
Paper Code:
ZOL-313
Credits:
3
Contact Hours:
45.00
Max. Marks:
100.00
Objective:
Course objectives:
This course will enable the students to -
1. Execute techniques used in biological sciences.
2. Examine working and applications of various types of Microscopic techniques.
3. Implement the working principles, instrumentation and applications of various analytical instruments like centrifugation, spectrophotometer, pH meter, Chromatography, Electrophoresis, PCR and ELISA.
4. Analyze the concepts of bioinformatics and its applications, learn to retrieve information from biological databases along with the submission of sequences into the databases and use of general computational tools in the field of Biology
Course Outcomes (COs):
9.00
Unit I:
UNIT I:
● Microscopy
● Magnification and Resolving Power
● Principle and Application of Microscopy
● Light (bright-field, dark-field and phase contrast)
● Electron (SEM and TEM)
● Fluorescence Microscope
● Spectrophotometer (UV- Visible)
12.00
Unit II:
UNIT II:
● Principles and use of analytical instruments:
● Centrifugation (Density and Differential)
● Chromatography (Paper and TLC)
● Electrophoresis (Agarose and PAGE)
● PCR
● ELISA
9.00
Unit III:
UNIT III:
● The Internet and the Biologists
● The Gene bank sequence database
● Structure data base
● Sequence analysis using GCG
10.00
Unit IV:
UNIT IV:
● Information retrieval from Biological database
● NCBI data model
● Sequence Alignment and database searching Practical aspects of multiple sequence alignment
● Phylogenetic analysis
5.00
Unit V:
UNIT V:
● Computer Basics
● MS-Office: MS. Word, Power Point and Excel
● Hardware and Software
● Multimedia
● Network Concepts(LAN,WAN)
● Internet
● Web Servers, Web Access
Essential Readings:
● Essential Bioinformatics, JinXiong, John Wiley and Sons. 2006.
● Introduction to Bioinformatics, A Teresa and D P Smith, Prentice Hall, 1999.
● Statistical Methods in Biology, N T J Bailey, Cambridge University Press, 1995.
● Statistics for Biologist, R C Campbell, Cambridge University Press, 1989.
● Bioinformatics, A practical Guide to the Analysis of Genes and Proteins, (2nd edition), A D Baxevanis, and B F Ouellette, John Wiley and Sons, 2002.
● Introduction to instrumental analysis-Robert Braun-McGraw Hill.
● A biologist Guide to principles and Techniques of Practical Biochemistry-K, Wilson and K.H. Goulding ElBSE dn.
● Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000.
● Clark &Swizer. Experimental Biochemistry. Freeman, 2000.
● Principles and Practice of Bioanalysis, R F Venn, Taylor and Francis, 2003.
● Locquin and Langeron. Handbook of Microscopy. Butterwaths, 1983
References:
SUGGESTED READINGS:
● Fundamentals of Biostatistics, Khan, Publishing Corporation, 1999
● Biostatistics: A Foundation for Analysis in Health Sciences, (6th edition), W W Daniel, John Wiley and Sons Inc., 1995.
● Instant notes, Bioinformatics, Westhead, Parish, and Twyman, (1stedition), Bios Scientific Publishers Ltd., 2003.
● Introduction to Bioinformatics, A M Lesk, Oxford University Press, 2002.
● Molecular databases for Protein sequence and Structure studies, J A Sillince and M Sillince, Springer Verlag, 1991
● Practical statistics for Experimental Biologists, A C Swardlaw, John Wiley and sons Inc., 1985
● Sequence Analysis Primer, Gribskov and Devereux, Stockton Press, 1989
● David W. Mount's "Bioinformatics" [Cold Spring Harbor Press; ISBN 0879697121].
● James Tisdall. Beginning Perl for Bioinformatics An Introduction to Perl for Biologists. Publisher: O'Reilly Media. October 2001.
e-RESOURCES:
JOURNALS:
● Journal of Bioinformatics and Computational Biology. ISSN (print): 0219-7200 | ISSN (online): 1757-6334.
● BMC Bioinformatics, Publisher: BioMed Central ltd. ISSN: 1471-2105.
Academic Year:
