Biological Tools and Techniques

Paper Code: 
ZOL-313
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 
Course objectives: 
This course will enable the students to - 
1. Execute techniques used in biological sciences. 
2. Examine working and applications of various types of Microscopic techniques. 
3. Implement the working principles, instrumentation and applications of various analytical  instruments like centrifugation, spectrophotometer, pH meter, Chromatography,  Electrophoresis, PCR and ELISA. 
4. Analyze the concepts of bioinformatics and its applications, learn to retrieve information from biological databases along with the submission of sequences into the databases and use of general computational tools in the field of Biology
 
Course Outcomes (COs):  
 

Course 

Learning outcomes 

(at course level)

Learning and  teaching strategies

Assessment  Strategies 

Paper  

Code

Paper Title

ZOL-313 

Biological  

Tools and  Techniques

The students will : 

CO 53:Examine various techniques  used in biological sciences. 

CO 54: Develop the functioning and  applications of various types of  Microscopic techniques. 

CO 55: Verify the working principles,  instrumentation and implement the  use of various analytical instruments  like centrifugation, spectrophotometer,  pH meter, Chromatography, 

Electrophoresis, PCR and ELISA. CO 56: Appraise about the concepts of  bioinformatics and its applications,  retrieve information from biological  databases along with the submission  of sequences into the databases and  will use general computational tools to solve research problems.

The approach in  teaching: 

Interactive Lectures,  Discussion, Tutorials,  Guest lectures,  PowerPoint  

presentations,  

Demonstration of  working of various  instruments 

Learning activities for  the students: 

Self learning  assignments, Effective  questions, Simulation,  Seminar presentation,  Giving tasks, 

● Class test 

● Semester  end  

examinatio 

ns, 

● Quiz,  

● Assignment s,  

● Presentatio n 

● Surprise  

test 

● Open book  test

 

9.00
Unit I: 
UNIT I:
● Microscopy
● Magnification and Resolving Power
● Principle and Application of Microscopy
● Light (bright-field, dark-field and phase contrast)
● Electron (SEM and TEM)
● Fluorescence Microscope
● Spectrophotometer (UV- Visible)
12.00
Unit II: 
UNIT II:
● Principles and use of analytical instruments:
● Centrifugation (Density and Differential)
● Chromatography (Paper and TLC)
● Electrophoresis (Agarose and PAGE)
● PCR
● ELISA
9.00
Unit III: 
UNIT III:
● The Internet and the Biologists
● The Gene bank sequence database
● Structure data base
● Sequence analysis using GCG
10.00
Unit IV: 
UNIT IV:
● Information retrieval from Biological database
● NCBI data model
● Sequence Alignment and database searching Practical aspects of multiple sequence alignment
● Phylogenetic analysis 
 
5.00
Unit V: 
UNIT V:
● Computer Basics
● MS-Office: MS. Word, Power Point and Excel
● Hardware and Software
● Multimedia
● Network Concepts(LAN,WAN)
● Internet 
● Web Servers, Web Access
 
Essential Readings: 
Essential Bioinformatics, JinXiong, John Wiley and Sons. 2006.
Introduction to Bioinformatics, A Teresa and D P Smith, Prentice Hall, 1999.
Statistical Methods in Biology, N T J Bailey, Cambridge University Press, 1995.
Statistics for Biologist, R C Campbell, Cambridge University Press, 1989.   
Bioinformatics, A practical Guide to the Analysis of Genes and Proteins, (2nd edition), A D Baxevanis, and B F Ouellette, John Wiley and Sons, 2002.
Introduction to instrumental analysis-Robert Braun-McGraw Hill. 
A biologist Guide to principles and Techniques of Practical Biochemistry-K, Wilson and  K.H. Goulding ElBSE dn. 
Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000. 
Clark &Swizer. Experimental Biochemistry. Freeman, 2000. 
Principles and Practice of Bioanalysis, R F Venn, Taylor and Francis, 2003. 
Locquin and Langeron. Handbook of Microscopy. Butterwaths, 1983 
 
 
 
 
References: 
SUGGESTED READINGS:
 
Fundamentals of Biostatistics, Khan, Publishing Corporation, 1999
Biostatistics: A Foundation for Analysis in Health Sciences, (6th edition), W W Daniel, John Wiley and Sons Inc., 1995.
Instant notes, Bioinformatics, Westhead, Parish, and Twyman, (1stedition), Bios Scientific Publishers Ltd., 2003.
Introduction to Bioinformatics, A M Lesk, Oxford University Press, 2002.
Molecular databases for Protein sequence and Structure studies, J A Sillince and M Sillince, Springer Verlag, 1991
Practical statistics for Experimental Biologists, A C Swardlaw, John Wiley and sons Inc., 1985
Sequence Analysis Primer, Gribskov and Devereux, Stockton Press, 1989
David W. Mount's "Bioinformatics" [Cold Spring Harbor Press; ISBN 0879697121].
James Tisdall. Beginning Perl for Bioinformatics An Introduction to Perl for Biologists.  Publisher: O'Reilly Media. October 2001.
 
e-RESOURCES: 
 
 
JOURNALS:
 
Journal of Bioinformatics and Computational Biology. ISSN (print): 0219-7200 | ISSN (online): 1757-6334.
BMC Bioinformatics, Publisher: BioMed Central ltd. ISSN: 1471-2105.
 
Academic Year: