Coming soon
Biological Tools and Techniques
Course objectives:
This course will enable the students to -
- Execute techniques used in biological sciences.
- Examine working and applications of various types of Microscopic techniques.
- Implement the working principles, instrumentation and applications of various analytical instruments like centrifugation, spectrophotometer, pH meter, Chromatography, Electrophoresis, PCR and ELISA.
- Analyze the concepts of bioinformatics and its applications, learn to retrieve information from biological databases along with the submission of sequences into the databases and use of general computational tools in the field of Biology
Course Outcomes (COs):
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Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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ZOL-313 |
Biological Tools and Techniques |
The students will : CO 53:Examine various techniques used in biological sciences. CO 54: Develop the functioning and applications of various types of Microscopic techniques. CO 55: Verify the working principles, instrumentation and implement the use of various analytical instruments like centrifugation, spectrophotometer, pH meter,Chromatography, Electrophoresis, PCR and ELISA. CO 56: Appraise about the concepts of bioinformatics and its applications, retrieve information from biological databases along with the submission of sequences into the databases and will use general computational tools to solve research problems.
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The approach in teaching: Interactive Lectures, Discussion, Tutorials, Guest lectures, PowerPoint presentations, Demonstration of working of various instruments
Learning activities for the students: Self learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks,
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- Microscopy
- Magnification and Resolving Power
- Principle and Application of Microscopy
- Light (bright-field, dark-field and phase contrast)
- Electron (SEM and TEM)
- Fluorescence Microscope
- Spectrophotometer (UV- Visible)
- Principles and use of analytical instruments:
- Centrifugation (Density and Differential)
- Chromatography (Paper and TLC)
- Electrophoresis (Agarose and PAGE)
- PCR
- ELISA
- The Internet and the Biologists
- The Gene bank sequence database
- Structure data base
- Sequence analysis using GCG
● Information retrieval from Biological database
● NCBI data model
● Sequence Alignment and database searching Practical aspects of multiple sequence alignment
Phylogenetic analysis
- Computer Basics
- MS-Office: MS. Word, Power Point and Excel
- Hardware and Software
- Multimedia
- Network Concepts(LAN,WAN)
- Internet
- Web Servers, Web Access
- Wilson and Walker. Practical Biochemistry. Cambridge, 2000.
- Cooper. The Cell-A Molecular Approach. ASM, 1997
- Sharma, Munjal and Shankar. A text book of Bioinformatics. Rastogi Publications, Meerut, UP.
- Essential Bioinformatics, Jin Xiong, John Wiley and Sons. 2006.
- Introduction to instrumental analysis-Robert Braun-McGraw Hill.
- A biologist Guide to principles and Techniques of Practical Biochemistry-K, Wilson and K.H. GouldingElBSEdn.
- Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000.
- Clark &Swizer. Experimental Biochemistry. Freeman, 2000.
- Principles and Practice of Bioanalysis, R F Venn, Taylor and Francis, 2003.
- Locquin and Langeron. Handbook of Microscopy. Butterwaths, 1983
- Boyer. Modern Experimental Biochemistry. Benjamin, 1993
- Freifelder. Physical Biochemistry. Freeman, 1982.
- John R.W. Masters. Animal Cell culture- A practical approach. IRL Press.
- Robert Braun. Introduction to instrumental analysis. McGraw Hill
- David W. Mount's "Bioinformatics" [Cold Spring Harbor Press; ISBN 0879697121].
- James Tisdall. Beginning Perl for Bioinformatics An Introduction to Perl for Biologists. Publisher: O'Reilly Media. October 2001.
