Biochemistry

Paper Code: 
ZOL 124
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course objectives:

This course will enable the students to –

  1. Enlighten the students about the importance of biological macromolecules, structures and configurational properties.
  2. Examine the key role of biochemistry in the metabolic process.

 

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL 124

Biochemistry

The students will –

CO17: Analyse the concepts of biocatalysts, enzyme activity and its kinetics.

CO18: Acquire detailed knowledge about the structure, classification, physico-chemical properties and biological significance of carbohydrates, amino acids, proteins and lipids.

CO19: Illustrate the anabolic and catabolic pathways of different cellular biomolecules, rate-limiting steps and energetic pathways.

CO20: Correlate the defective enzymes and inborn errors of metabolism.

CO21: Assess the role of biomolecules in the metabolic processes.

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

Class test

Semester end examinations, Quiz,

Assignments,

Presentation

Individual and group projects

 

12.00
Unit I: 
UNIT I
• Biocatalysis
Classification and nomenclature of enzymes
Mechanism of enzyme action
Enzyme Kinetics
Inhibition of enzyme
Isoenzymes and coenzymes
Energy consideration
● Biological oxidation and reduction
Fundamental reactions of biological oxidation
Redox potential and Electron transfer system enzymes
 

 

12.00
Unit II: 
UNIT II
Carbohydrates
 
Definition, general properties, classification and configuration of carbohydrates
Metabolism
Catabolism of glucose with Stoichiometry, Glycolysis, Krebs cycle and Cori’s cycle
Phosphogluconate pathways (Pentose Phosphate Pathway) Glycogenesis, Glycogenolysis and Gluconeogenesis
Inborn errors of metabolism: Galactosemia, Lactose Intolerance, Glycogen storage diseases.
 
12.00
Unit III: 
UNIT III
• Lipids
Definition, general properties and classification
Structure and importance of-:
Simple lipids- fats and wax
Compound lipids- phospholipids, glycolipids, amino lipids and sulpholipids
Steroids- cholesterol, bile acids, steroid hormones
• Lipoproteins
• Lipid Metabolism
Beta oxidation andLipogenesis.
Inborn errors of metabolism: Niemann pick disease,TaySach’sdisease,Gaucher’s disease, Krabbe’s disease
 
15.00
Unit IV: 
UNIT IV
  • General properties and classification of proteins
  • Amino acid structure and classification
  • Structure of protein-Primary, Secondary, Tertiary And Quaternary
  • Ramachandran plots
  • Globular and fibrous protein- motif in globular proteins
  • Protein Sequencing

 

10.00
Unit V: 
UNIT V
  • Protein metabolism    
  • Amino acid degradation: Deamination reaction- Oxidative and non oxidative deamination reaction, Transamination and Trans deamination, Decarboxylation, Ornithine cycle of urea formation, Fate of carbon skeleton of amino acids(Metabolism of individual amino acid excluded)
  • Inborn errors of metabolism: Alkaptonuria, Phenylketonuria, Maple Syrup, Tyrosinemia, Albinism, Urea Disorders.

 

Essential Readings: 

ESSENTIAL READINGS:

  • U. Satyanaryana . Biochemistry IV Edition, Elsevier (2013)
  • Jain,J.L. Fundamentals of Biochemistry ,S.Chand publishers New Delhi.
  • Medical Physiology; Chatterjee, M.N and Shinde, R.; Jaypee brothers.(2007)
  •  Berg, J. M., Tymoczko, J. L. and Stryer, L. (2006). Biochemistry. VI Edition. W.H Freeman  and Co.
  •  Lehninger, A.D. Principles of Biochemistry, CBS Publishers and Distributors, Shahdara, Delhi. ● Nelson, D. L., Cox, M. M. and Lehninger, A.L. (2009). Principles of Biochemistry. IV Edition.  W.H. Freeman and Co

 

References: 
SUGGESTED READINGS:
● Harvey, Richard A., Ph. D. (2011). Lippincott's illustrated reviews: Biochemistry. Philadelphia :Wolters Kluwer Health
● Tortora, G.J. and Derrickson, B.H. (2009). Principles of Anatomy and Physiology, XII Edition,  John Wiley & Sons, Inc. 
● Principles of Biochemistry; Smith et.al; McGraw Hill, New York. 
● Textbook of Medical Biochemistry; Ramakrishnan, S. Prasanan, K.G and Rajan, R; Orient  Longman, Madras 
● Conn, E.E. and Stuff, P.K. Outlines of Biochemistry,John Wiley New York.  
● Donald Voet and Judith G. Voet Biochemistry, John Wiley and Sons., New York.  
● H.S. Srivastava, Element of Biochemistry, Rastogi Publications Meerut.  
 
 
E-RESOURCES: 
● e-PGPATHSHALA-INFLIBNET CENTRE-
● Biochemistry- Swayam Prabha-https://dth.ac.in/medical/course.php
 
e-JOURNAL: 
● Journal of Biochemistry- ISSN 1756-2651
● Biochemistry- ACS Publication- ISSN 1520-4995
● Journal of Cellular Biochemistry- Wiley online Library- Online ISSN: 1097-4644
 
Academic Year: 
2022-2023
  • Printer-friendly version
  • PDF version