BIOCHEMISTRY

Paper Code: 
ZOL 124
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course objectives:

This course will enable the students to -

  1. This course will enlighten the students about the importance of biological macromolecules, structures and configurational properties.
  2. This course also provides the students with an appreciation and an understanding of key metabolic biochemistry concepts.

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL 124

Biochemistry

Students will be able to:

CO12: Understand the concepts of biocatalysts,    enzyme activity and its kinetics.

CO13: Acquire the detailed knowledge about the structure, classification, physico- chemical properties and biological significance of carbohydrates, amino acids, proteins and lipids.

CO14: Illustrate the anabolic and catabolic pathways of different cellular biomolecules, rate-limiting steps and energetic pathways.

CO15: Correlate the defective enzymes and inborn errors of metabolism.

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

 

Class test

Semester end examinations, Quiz,

Assignments,

Presentation

Individual and group projects

 

10.00
Unit I: 
UNIT I

Biocatalysis

  •     Classification and nomenclature of enzymes
  •     Mechanism of enzyme action
  •     Enzyme kinetics
  •     Inhibition of enzym
  •     Isoenzymes and coenzymes

Energy consideration

  •     Biological oxidation and reduction
  •     Fundamental reactions of biological oxidation
  •     Redox potential and Electron transfer system enzymes
14.00
Unit II: 
UNIT II

Carbohydrates

    Definition , general properties, classification and configuration of carbohydrates

Metabolism

    Catabolism of glucose with Stoichiometry, Glycolysis , Krebs cycle and Cori’s cycle

    Phosphogluconate pathways(Pentose Phosphate Pathway)

Glycogenesis, Glycogenolysis and Gluconeogenesis

Inborn errors of metabolism: Galactosemia, Lactose Intolerance, Glycogen storage diseases.

12.00
Unit III: 
UNIT III

Lipids

    Definition, general properties and classification

    Structure and importance of -:

        Simple lipids- fats and wax

        Compound lipids- phospholipids, glycolipids, amino lipids and sulpho lipids

        Steroids- cholesterol, bile acids, steroid hormones

        Lipoproteins

        Lipid Metabolism- Beta oxidation and Lipogenesis.

        Inborn errors of metabolism: Niemann pick disease, TaySach’sdisease, Gaucher’s disease, Krabbe’s disease

14.00
Unit IV: 
UNIT IV

General properties and classification of proteins

  •         Amino acid structure and classification
  •         Structure of protein-Primary, Secondary, Tertiary and Quaternary
  •         Ramachandran plots
  •         Globular and fibrous protein- motif in globular proteins
  •          Protein sequencing
10.00
Unit V: 
UNIT V

Protein metabolism

    Amino acid degradation:Deamination reaction- Oxidative and non oxidative deamination reaction, Transamination and Transdeamination, Decarboxylation, Ornithine cycle of urea formation , Fate of carbon skeleton of amino acids(Metabolism of individual amino acid excluded)

Inborn errors of metabolism:  Alkaptonuria, Phenylketonuria, Maple Syrup, Tyrosinemia, Albinism ,Urea Disorders.

Essential Readings: 

 Essential readings:
 

  • Biochemistry, D.Voet and J.G.Voet, John Wiley & Sons, (2nd Edition), 1995.
  • Principles of Biochemistry, Lehninger ,Nelson and Cox, (3rd Edition), 1999.
  • Biochemisty, U. Satyanarayan and U. Chakrapani (4th edition)
  • Text Book of Medical Physiology; Chatterjee, M.N and Shinde, R.; Jaypee brothers.

Suggested Readings:
 

  • Outlines of Biochemistry; Conn, E.E. and Stumpf P.K. ; John Wiley, New York.
  • Principles of Biochemistry; Smith; McGraw hill, New York.
  • Biochemistry; Styrer, L; Freeman Publications.
  • Textbook of Medical Biochemistry; Rama krishnan, S. Prasanan, K.G and Rajan, R.: Orient Longman, Madras
  • Principles and Techniques in Biochemistry and Molecular Biology, Edited by Keith Wilson and John Walker, Cambridge University Press, (6th Edition), 2007.
  • Biochemistry and Molecular Biology by W.H. Elliot and Daphne C. Elliot, Oxford University Press, (3rd Edition), 2005.
  • Biochemistry, J. David Rawn, Panima Publishing Corporation, 1st Indian Reprint, 2004.
  • Understanding Chemistry, CNR Rao, University Press, Hyderabad 1999.
  • Essentials of Molecular Biology, David Friefelder, Jones and Barllett Publications,7th Reprint, 2001.
  • Instant Notes- Biochemistry (1st Indian Edition), B.D. Hames and N.M. Hooper, Viva Books Pvt, Ltd., 2001.
Academic Year: 
2020-2021
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