BASICS OF MOLECULAR BIOLOGY PRACTICAL

Paper Code: 
25CZOL514
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
This course will enable the students to
1. Gain hands-on experience with molecular biology techniques like gel electrophoresis.
2. Examine Polytene Chromosomes and estimate DNA.
3. Enhance understanding of quantitative estimation of RNA and DNA, and interpret electron micrographs of DNA replication and transcription.
 
Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessmen t Strategies

Course Code

Course Title

 

 

 

 

 

 

25CZOL514

 

 

 

 

 

 

Practical

CO:115 Get hands-on experience of various molecular biology techniques including gel electrophoresis and their applications in the research field.

CO:116 Enhance their knowledge about Quantitative estimation of RNA

and DNA

CO:117 Interpretation of electron micrographs of DNA replication and Transcription

CO:118 Quantitatively estimate RNA using the Orcinol reaction. Interpret electron micrographs of DNA replication, transcription, and split genes.

CO:119 Demonstrate skills and record the concepts in the form of practical files and reflect on personal learning experiences and experimental outcomes during viva voce.

CO:120 Contribute effectively in course-specific interaction

The approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Guest lectures

Learning activities for the students: Self-learning, assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Regular activities like quiz, surprise test, explanation of given task

 

Semester end examinatio ns,

Quiz, Assignment, Presentation Surprise test Open book test

 

1. Study of Polytene chromosomes from Chironomous / Drosophila larvae
2. Isolation of Eukaryotic DNA
3. Quantitative estimation of salmon sperm/calf thymus DNA using colorimeter (Diphenylamine reagent) or spectrophotometer (A260 measurement)
4. Quantitative estimation of RNA using Orcinol reaction
5. Study and interpretation of electron micrographs/ photograph showing
(a) DNA replication
(b) Transcription
(c) Split genes
 
 

 

Essential Readings: 
● Practical Zoology, vertebrates, Lal, S.S. Rastogi Publications, Meerut
● Introductory practical Biochemistry, Sawhney S.K., Singh R. 2009. 7th edition, Narosa publishing house Pvt. Ltd.
● Jordon, E.L., Verma P.S. Invertebrate Zoology, S Chand and Co.
● Laboratory manual in Invertebrate & Experimental Zoology, Jacob Vyas Ramesh Book Depot, New Delhi.
● Practical Exercises in Cytology, Genetics, Plant Breeding and Biostatistics. Raghuvanshi.
 
References: 
● Zubay G. Biochemistry, William C. Brown Pub. Standard methods of biochemical analysis. Thimmaiah, S.R.
Kalyani Publishers, Noida
e-RESOURCES:
 
SCHEME FOR PRACTICAL EXAMINATION:
Scheme for CA End Practical Examination
Exercise 1 Exercise II File Viva Attendance Total
5 5 10 5 5 30
Scheme for Semester End Practical Examination
Exercise I Exercise II Exercise III Exercise IV Spotting File Viva Total
8 8 8 8 6x3=18 10 10 70
 
Academic Year: 
2025-2026
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