ANIMAL BIOTECHNOLOGY

Paper Code: 
24DZOL615B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 
Course Objectives
This course will enable the students to –
1. Analyse the fundamental molecular tools and their applications
2. Appraise strategies of genetic engineering for possible applications in the biotechnology
3. Envisage the significance of genetic modifications or manipulations.
 
Course Outcomes: 

Course

Learning outcomes

(at course level)

Learning and 

teaching strategies

Assessment

Strategies

Course Title

 

Animal

Biotechnology

THEORY

 

CO:181 Examine the basic  concepts in Genetic Engineering and enzymes involved .

CO:182 Explain various Cloning Vectors, Transformation Techniques and Transduction

CO:183 DeducevariousDNA libraries,blotting techniques, DNA probes and DNA sequencing methods

CO:184 Describe the science  of biotechnology and biotechnology methods  for construction transgenic animals

CO :185 Outline animal cell culture, Expressing cloned genes in mammalian cells, 

Molecular diagnosis of genetic diseases and role of Recombinant DNA in medicines

CO:186Contribute effectively in course-specific interaction

The approach in  teaching: Interactive Lectures,  Discussion, 

Tutorials, Reading  assignments, 

Demonstration, 

Team teaching

 

Learning activities for the students: Self-learning assignments, 

Effective questions,  Simulation,

Assessment 

Strategies

Class test, 

Semester end 

examinations, 

Quiz, Solving 

problems in 

tutorials, 

Assignments, 

Presentation.

 

 

10.00
Unit I: 
Introduction
Basic Concept in Genetic Engineering 
Enzymes used in Genetic Engineering: Restriction enzymes: Nomenclature, Types 
DNA Ligase  
End Modification Enzymes 
 
14.00
Unit II: 
Molecular Techniques in Gene manipulation
 Cloning vectors: Plasmids, Cosmids, Lambda Bacteriophage, M13, BAC, YAC,  
Transformation techniques: Calcium chloride method and electroporation. 
Transduction  
 
12.00
Unit III: 
DNA libraries and sequencing techniques
 Construction of genomic and cDNA libraries and screening by colony and plaque  hybridization 
Southern, Northern and Western blotting 
 DNA sequencing: Sanger method, Polymerase Chain Reaction, 
DNA Probes
12.00
Unit IV: 
Genetically Modified Organisms
Production of cloned and transgenic animals: Nuclear Transplantation, Retroviral Method,  DNA microinjection  
Applications of transgenic animals: Production of pharmaceuticals, production of donor  organs, knock out mice.  
 
12.00
Unit V: 
Culture Techniques and Applications
Animal cell culture, Expressing cloned genes in mammalian cells,  
Molecular diagnosis of genetic diseases (Cystic fibrosis, Sickle cell anemia)  
Recombinant DNA in medicines: Recombinant insulin and human growth hormone, Gene therapy  
 
Essential Readings: 
SUGGESTED READINGS  
• Brown, T.A. (1998). Molecular Biology Labfax II: Gene Cloning and DNA Analysis. II  Edition, Academic Press, California, USA.  
• Glick, B.R. and Pasternak, J.J. (2009). Molecular Biotechnology - Principles and  Applications of Recombinant DNA. IV Edition, ASM press, Washington, USA. 
• Griffiths, A.J.F., J.H. Miller, Suzuki, D.T., Lewontin, R.C. and Gelbart, W.M. (2009). An  Introduction to Genetic Analysis. IX Edition. Freeman and Co., N.Y., USA. Snustad, D.P.  and Simmons, M.J. (2009). Principles of Genetics. V Edition, John Wiley and Sons Inc. 
Essential Readings
• Watson, J.D., Myers, R.M., Caudy, A. and Witkowski, J.K. (2007). Recombinant DNA Genes and Genomes- A Short Course. III Edition, Freeman and Co., N.Y.,USA. 
• Beauchamp, T.I. and Childress, J.F. (2008). Principles of Biomedical Ethics. VI Edition,  Oxford University Press.
 
Academic Year: 
2024-2025
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