Animal Biotechnology

Paper Code: 
DZOL601B T
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

 

DZOL601B T

Animal Biotechnology

The students will be able to-

 

CO1: Examine the basic concepts in Genetic Engineering and Cloning.

 

CO2: Deduce the use of Biotechnology in Medicine

CO3: Describe the science of biotechnology and

biotechnology methods used to conduct experiments and develop products for bioethical use.

 

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

Class test

Semester end examinations,

Quiz

Assignments

Presentation

Individual and group projects

 

10.00
Unit I: 
UNIT I
Introduction     
Basic Concept in Genetic Engineering
Enzymes used in Genetic Engineering: Restriction enzymes: Nomenclature, Types
DNA Ligase 
End Modification Enzymes
14.00
Unit II: 
Unit II
Molecular Techniques in Gene manipulation                   
Cloning vectors: Plasmids, Cosmids, Lambda Bacteriophage, M13, BAC, YAC, 
Transformation techniques: Calcium chloride method and electroporation.
Transduction 
12.00
Unit III: 
Unit III
DNA libraries and sequencing techniques
Construction of genomic and cDNA libraries and screening by colony and plaque hybridization Southern, 
Northern and Western blotting 
DNA sequencing: Sanger method Polymerase Chain Reaction,
DNA Probes 
12.00
Unit IV: 
Unit IV
Genetically Modified Organisms                                 
Production of cloned and transgenic animals: Nuclear Transplantation, Retroviral Method, DNA microinjection 
Applications of transgenic animals: Production of pharmaceuticals, production of donor organs, knock out mice. 
 
12.00
Unit V: 
Unit V
Culture Techniques and Applications                             
Animal cell culture, Expressing cloned genes in mammalian cells, 
Molecular diagnosis of genetic diseases (Cystic fibrosis, Sickle cell anemia) 
Recombinant DNA in medicines: Recombinant insulin and human growth hormone,
Gene therapy 
 
Academic Year: 
2023-2024
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