Animal Biotechnology: Practical

Paper Code: 
DZOL602B P
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

DZOL602B P 

 

Animal Biotechnology: Practical

The students will be able to-

 

CO1: Correlate the

principles and working of

various instruments in

biotechnology laboratory

CO2: Isolate the genomic and plasmid DNA 

CO3: Conduct Restriction digestion 

CO4: Gain the knowledge of blotting techniques

 

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

Class test

Semester end examinations,

Quiz

Assignments

Presentation

Individual and group projects

 

1. Genomic DNA isolation from E. coli
2. Plasmid DNA isolation (pUC 18/19) from E. coli 
3. Restriction digestion of plasmid DNA.
4. 4.Principle and working of equipment used in laboratory (Laminar Air Flow, Autoclave, Incubator, Electrophoresis chamber)
5. To study following techniques through photographs a. Southern Blotting b. Northern Blotting c. Western Blotting d. DNA Sequencing (Sanger's Method) 
6. Project report on animal cell culture
 
Essential Readings: 
 
 SUGGESTED READINGS  
● Brown, T.A. (1998). Molecular Biology Labfax II: Gene Cloning and DNA Analysis. II Edition, Academic Press, California, USA.  
● Glick, B.R. and Pasternak, J.J. (2009). Molecular Biotechnology - Principles and Applications of Recombinant DNA. IV Edition, ASM press, Washington, USA.  
● Griffiths, A.J.F., J.H. Miller, Suzuki, D.T., Lewontin, R.C. and Gelbart, W.M. (2009). An Introduction to Genetic Analysis. IX Edition. Freeman and Co., N.Y., USA.  
Academic Year: 
2023-2024
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