Molecular Biology

Paper Code: 
ZOL-213
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

Objectives

The course objectives to provide students with a basic understanding of:

  1. Structure and packing of genetic material in the nucleus.
  2. How the DNA in a genome is organized, replicated, and repaired
  3. How genetic information in the DNA is selectively expressed as functional proteins.
  4. How genetic information transferred in the forms of codons and post translational modifications.

Learning outcomes- By the end of the course, students will be able to:

  1. Describe the Structure and packing of genetic material in the nucleus.
  2. Compare different chromosomes according to their structure and packing in the nucleus.
  3. Describe the structure and types of Nucleic acids
  4.  Describe the process and importance of DNA replication.
  5. Describe the process of Transcription and translation in the cell.
9.00

Chromosomes: Morphology, Chromonema, Chromomeres, Telomeres, Primary & secondary constrictions, Chromatids

Chromosome type- Polytene&Lampbrush

Chromosomal Organization: Euchromatin, Heterochromatin , Nucleosome concept

7.00

Nucleosides, Nucleotides

DNA

DNA structure(A, B, Z type)

RNA structure & types( mRNA, rRNA, tRNA)

 

10.00

Replication of prokaryotes (Semi-conservative mechanism: Experiments of Messelson& Stahl)

Prokaryotic Polymerases, Topoisomerases, Single stranded binding protein, Replication forks(Unidirectional & Bidirectional), Leading & lagging strand, RNA primers & Okazaki fragments

Brief Idea of Eukaryotic Replication( Ori sites and Enzymes involved)

10.00

Prokaryotic Transcription

  • RNA Polymerases
  • Mechanism of transcription

Brief idea about transcription factors in eukaryotic transcription      

Post Transcriptional modification in Eukaryotes

  • Capping
  • Polyadenylation

Splicing

9.00

Genetic Code

Prokaryotic translation

Translational Machinery

Translational Initiation, Elongation and termination

Essential Readings: 

Essential Readings:

  • Cell Biology by C.B. Powar; Himalaya Publishing House.
  • Cell and Molecular Biology; Phillip Sheeler   Donald E. Bianchi; John Wiley and Sons.
  • The Cell: A Molecular Approach. Cooper, G. Houseman G.R. SinaeurAssoc, New York.
References: 

Suggested Reading

  • Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009).The World of the Cell.VII Edition. Pearson Benjamin Cummings Publishing, San
  • Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts,
  • Peter Walter: Molecular Biology of the Cell, IV Edition.
  • Cooper G. M. and Robert E. Hausman R. E. The Cell: A Molecular Approach, V Edition, ASM Press and Sinauer Associates.
  • De Robertis, E.D.P. and De Robertis, E.M.F. (2006).Cell and Molecular Biology.VIII Edition. Lippincott Williams and Wilkins, Philadelphia.
  • Karp, G. (2010) Cell and Molecular Biology: Concepts and Experiments. VI Edition.John Wiley and Sons.Inc.
  • Lewin B. (2008). Gene XI, Jones and Bartlett
  • McLennan A., Bates A., Turner, P. and White M. (2015).Molecular Biology IV Edition.GS, Taylor and Francis Group, New York and London.
  • Molecular Biology of the gene; Watson J.D.; Benzamin/ Cummings.
  • Molecular Cell biology (2nd Edition) J. Darnell, H. Lodish and D.Baltimore, Scientific American Books,USA,1994.
  • Cell & Molecular Biology, (8th edition), E D P Roberties& E M F Roberties,  Lippincott Williams & Wilkins, 2005
  • Cell and Molecular Biology, (3rd edition), P Sheeler and D E Bianchi, John Wiley & Sons, Inc, 1987
  • The cell. A Molecular Approach, (4th edition), G H Cooper and R E Hausman, ASM Press, 2007
Academic Year: 
2019-2020
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