Coming soon
Biotechnology and Immunology
Course Objectives:
This course will enable the students to
1. Acquire basic understanding of tools used in molecular genetics, and their potential applications to life sciences.
2. Analyze basic concepts and techniques in genetic engineering and Cloning.
3. Develop an understanding of biotechnology in medicine.
4. Illustrate the ability of our immune system to defend against invading pathogens, concepts of immunity, hypersensitivity, autoimmunity and vaccination
Course Outcomes (COs):
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Course |
Learning outcomes (at course level) |
Learning and teaching strategies |
Assessment Strategies |
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Paper Code |
Paper Title |
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ZOL 501
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Biotechnology and Immunology |
The students will- CO50: Evaluate the tools used in molecular genetics, and their potential applications to life sciences. CO51: Examine the basic concepts in Genetic Engineering and Cloning. CO52: Deduce the use of Biotechnology in Medicine CO53: Conceptualize how the innate and adaptive immune responses coordinate to fight invading pathogens. CO54: Predict how immunomodulatory strategies can be used to enhance immune responses or to suppress unwanted immune responses such as might be required in hypersensitivity reactions, transplantations or autoimmune diseases. |
Approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching Learning activities for the students: Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical |
Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects |
- Basic concepts in Genetic Engineering
- Enzymology of Genetic engineering- Restriction enzymes, DNA ligase
- Cloning vehicles-Plasmids, Cosmids, Lambda phage and Yeast plasmids
- Analysis & expression of Cloned gene in host cell, Southern blotting, Northern blotting, Polymerase chain reaction, DNA probes
- Genomic library and cDNA library
- Methods of Mice Transgenesis, Applications in brief.
- Hybridoma technology, Monoclonal antibodies and their applications
- Brief account of Cloning: Genomic research-its advantages and disadvantages
- Elementary idea of Human Genome Project
Immunity
- Innate & adaptive immunity
- Cells, Tissues and organs of immune system
- Mechanism of Humoral and Cell mediated immune response
- MHC – Class I and Class II Structure and Function
● Antigen & antibodies- Structure, types
● Cytokines-types, properties and function
● Complement system and its action (classical and alternative pathway)
● AIDS- causes, symptoms, treatment, mode of infection, HIV life cycle
BOOKS RECOMMENDED:
● Principles of gene manipulation; An introduction to genetic engineering; Old, R.W and Primrose, S.B; Blackwell Science.
● Elementary biotechnology, Gupta, P.K; Rastogi Publications; Meerut.
● Immunology; Kuby; W.H; Freeman Publications.
● R.C. Dubey, A TextBook of Biotechnology, S. Chand and Company, New Delhi.
● H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd. New Delhi.
● Molecular Biotechnology. Glick B.R. & Pasternak J. J; ASM Press.
● D.M. Wear, Immunology ELBS (English) Language Book Society Low Priced edition, Longman Singapore Publishers Ltd.
● Iyan M. Roitt Essential Immunology ELBS Low Priced Edition Black well Scientific Publications, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006). Immunology, VI Edition. W.H. Freeman and Company.
● David, M., Jonathan, B., David, R. B. and Ivan R. (2006). Immunology,VII Edition, Mosby, Elsevier Publication.
● Abbas, K. Abul and Lechtman H. Andrew (2003.) Cellular and Molecular Immunology. V Edition. Saunders Publication.
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