Molecular Biology & Genetics II

Paper Code: 
ZOL 202
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

Course Objectives:  

This course will enable the students to

  1. Correlate the basic concepts of molecular biology and genetics
  2.  Develop understanding of structure and packing of genetic material in the nucleus.
  3. Illustrate how DNA in a genome is organized, replicated, and repaired.
  4. Critically analyze how genetic information in the DNA is selectively expressed as functional proteins.
  5. Distinguish Chromosomes involved in Sex determination, chromosomal      aberrations and Mutations and their consequences.

Course Outcomes (COs):

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

 

 

 

ZOL 202

Molecular Biology & Genetics II

The students will –

CO19: Simulate the structure and packing of genetic material in the nucleus.

CO20: Analyze the structure and types of Nucleic acids CO21: Correlate the process and           importance           of DNA replication, Transcription and translation in the cell.

CO22: Deduce the concept of chromosomes involved in sex determination

CO23: Formulate how natural polymorphism and genetic variation can give rise to mutant genes, and how these genetic errors are inherited.

 

Approach in teaching:

Class Lectures Seminars, Discussion, Tutorials, Reading assignments, Demonstration,

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

Group discussion Class test, Semester end examinations Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects

 

9.00
Unit I: 
UNIT I

 

        Chromosomes:

  • Morphology, Telomeres, Primary & secondary constrictions, Chromatids
  • Chromosome type- Polytene and Lampbrush
  • Chromosomal Organization: Euchromatin and heterochromatin
  • Nucleosome concept
7.00
Unit II: 
UNIT II

                

            DNA

 

  • DNA structure(A, B, Z type)
  • Experiments of Meselson & Stahl
  • Replication(Semi-conservative mechanism) of prokaryotes with reference to polymerases
  • Topoisomerases, Single stranded binding protein, Replication forks(Unidirectional & Bidirectional), Leading & lagging strand, RNA primers & Okazaki fragments

 

10.00
Unit III: 
UNIT III

 

           RNA

  •  Structure & types (mRNA, rRNA, tRNA)
  • Transcription of prokaryotes (Idea about Polymerases)
  • Translation – Basic idea about Protein synthesis of prokaryotes
  • Genetic code
10.00
Unit IV: 
UNIT IV

 

 

  • Determination of Sex – Chromosomal mechanism & environmental influence, the sexual function of X &Y chromosome, Gynandromorphs, Genic Balance Theory
  • Sex linked inheritance in man (Colour-blindness &Haemophilia)& Drosophila (Eye colour), Y linked genes, Sex limited genes, Sex influenced genes
9.00
Unit V: 
UNIT V

 

 ●    Mutations- Chromosomal aberrations, Gene mutations

●    Human genetics- Chromosomal & single gene disorder, Genetics counseling, Amniocentesis

●    Eugenics & Euthenics

 

Essential Readings: 

 

   BOOKS RECOMMENDED:

 

●    Cell Biology by C.B. Powar; Himalaya Publishing House

●    A Textbook of Genetics; Rastogi, V.B.; Ramnath Kedarnath.

●    Cell and Molecular Biology; Phillip Sheeler Donald E. Bianchi; John Wiley and Sons.

●    The Cell: A Molecular Approach. Cooper, G. Houseman G.R. SinaeurAssoc, New York.

●    Genetics; Winchester, A.M; Oxford and IBH Publishing Co.

●    Genetics; Strickberger; Macmillan, Prentice Hall of India.

●    Principles of Genetics; Gardener, E,J; Wiley eastern, New Delhi.

●    Primrose, S.B. Tyman, R.M. and Old, R.W. Principles of gene manipulation, Willey- Blacwell.

●    Cell and Molecular Biology; Gerald Karp; John Wiley and Sons,inc

●    Molecular Biology of the cell; Bruce Alberts, Julian Lewis, James D.Watson; Garland Publishings

●    Cell and Molecular Biology; Phillip Sheeler, Donald E. Bianchi; John Wiley and Sons. Becker, W.M., Kleinsmith, L.J., Hardin. J. and Bertoni, G. P. (2009). The World of the Cell. VII Edition. Pearson Benjamin Cummings Publishing, San

●    Bruce Alberts, Alexander Johnson, Julian Lewis, Martin Raff, Keith Roberts,

●    Peter Walter: Molecular Biology of the Cell, IV Edition.

●    Cooper G. M. and Robert E. Hausman R. E. The Cell: A Molecular Approach, V Edition, ASM Press and Sinauer Associates.

●    De Robertis, E.D.P. and De Robertis, E.M.F. (2006). Cell and Molecular Biology. VIII Edition. Lippincott Williams and Wilkins, Philadelphia.

●    Karp, G. (2010) Cell and Molecular Biology: Concepts and Experiments. VI Edition. John Wiley and Sons. Inc.

●    Lewin B. (2008). Gene XI, Jones and Bartlett

●    McLennan A., Bates A., Turner, P. and White M. (2015). Molecular Biology IV Edition. GS, Taylor and Francis Group, New York and London

 

Academic Year: 
2021-2022
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