Practical

Paper Code: 
ZOL-515
Credits: 
4
Contact Hours: 
120.00
Max. Marks: 
100.00
Objective: 

OBJECTIVES AND OUTCOMES

  • Design and conduct experiments, using a broad variety of experimental techniques, and interpret the data obtained to draw conclusions about whether the results do or do not support a hypothesis being tested and whether the experimental design was appropriate.
  • Work collaboratively with other students and with faculty, understanding that most scientific knowledge/conclusions draw(s) on multiple sources of data and multiple experimental approaches.
  • Learn “best approaches” for conducting experiments including laboratory safety, data replication and quality control, record keeping, and other aspects of “responsible conduct of research”.
  • Gain familiarity with an area of science such that the students can present their work to a professional audience and also discuss their work with a general audience.
  • Develop problem solving skills and analytical thinking skills.

 

 



Biotechnology

Principle and working of equipments used in biotechnology laboratory(SDS-PAGE apparatus, horizontal DNA Electrophoresis chamber, Laminar Airflow, Autoclave, Incubator, pH meter, Homogenizer, Ascending and Descending Chromatographic chamber, Thin Layer Chromatographic Apparatus, Microtome)

Microbiology:

·         Preparation and use culture media for microbes

·         Study of microbes in food material(curd, milk, rotten fruits and vegetables)

·         Educational tour to any Microbiology lab (Food processing factory, Distillery, Dairy etc.) Candidates will have to submit a report on it.

·         Culturing of Bacteria from soil/water samples.

·         Quality testing of milk

·         Disc diffusion assay

·         Spread plate and Streak Plate

 

Immunology:

Experiments on Antigen and Antibody reaction.

  • Blood typing
  • ELISA
  • Ouchterlony double diffusion

 

 

Essential Readings: 
  • Practical Biochemistry: Principles &Techniques; Keith Wilson& John Walker; Cambridge University Press.
  • Microbiology, A laboratory manual by Cappucino, James,G.
Academic Year: