Biotechnology & its Applications

Paper Code: 
ZOL-511
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

OBJECTIVES- The course objective is to provide students with a basic understanding of:

 

  1. The tools used in molecular genetics, and their potential applications to life sciences.
  2. Basic concepts in Genetic Engineering and cloning
  3. Techniques of genetic engineering, Hybridoma technology
  4. Genomic research and Elementary idea of Human Genome Project
  5. Use of Biotechnology in Medicine
  6. Molecular Markers (RFLP,RAPD,AFLP)

 

Learning outcomes-

By the end of the course, students will be able to:

 

Describe the tools used in molecular genetics, and their potential applications to life sciences.

  1. Describe the basic concepts in Genetic Engineering and cloning
  2. Describe the techniques of genetic engineering.
  3. Describe Genomic research and outcomes of Human Genome Project
  4. Describe use of Biotechnology in Medicine
  5. Describe the use of Molecular Markers (RFLP,RAPD,AFLP)
10.00
Unit I: 
UNIT I

Basic concepts in Genetic Engineering

  • Enzymology of Genetic engineering- Restriction enzymes, DNA ligase

Cloning vehicles-plasmids, cosmids, lambda phage & yeast plasmids

9.00
Unit II: 
UNIT II

Introduction of cloned genes into the host cells

  • Transformation
  • Transduction
  • Transgenic animals, their uses and biotechnology

Brief account of cloning

5.00
Unit III: 
UNIT III

Analysis & expression of cloned gene in host cell, Southern blotting, Northern blotting, Polymerase chain reaction, DNA probes

Genomic library &cDNA library

11.00
Unit IV: 
UNIT IV

Hybridoma technology, Monoclonal antibodies and their applications

Genomic research-its advantages and disadvantages

 Elementary idea of Human Genome Project

10.00
Unit V: 
UNIT V

Biotechnology in Medicine (outline idea only):

  • Antibiotics
  • Vaccines
  • Vitamins.
  • Artificial Blood.

Potential hazards and regulations of genetic engineering

Molecular Markers (RFLP,RAPD,AFLP)

Essential Readings: 
  • Principles of gene manipulation; An introduction to genetic engineering; Old, R.W & Primrose, S.B; Blackwell Science.
  • Elementary biotechnology, Gupta, P.K; RastogiPuplications; Meerut.
  • Immunology; Kuby; W.H; Freeman Publications.
  • R.C. Dubey, A Text Book of Biotechnology, S. Chand and Company, New Delhi.
References: 
  • H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd. New Delhi.
  • D.M. Wear, Immunology ELBS (English) Language Book Society Low Priced edition, Longman Singapore Publishers Ltd.
  • Iyan M. Roitt essential Immunology EIBS Low Priced Edition Black well Scientific Publications.
  • Molecular Biotechnology. Glick B.R. & Pasternak J. J; ASM Press.
Academic Year: 
2019-2020
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