Biological Tools and Techniques

Paper Code: 
ZOL- 313
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Objective: 

Objectives

The course objective is to provide students with a basic understanding of:

 

  1. Various techniques used in biological sciences
  2. Types of Microscope and their use.
  3. Principles and use of analytical instruments: centrifugae, spectrophotometer, pH meter.
  4. Chromatography and its types, Electrophoresis, PCR and ELISA
  5. Computational tools in the field of Biology
  6. Basics of Computer

 

Learning Outcomes- By the end of the course, students will be able to:

 

  1. Describe Various techniques used in biological sciences
  2. Applications of various types of Microscope.
  3. Describe and use various analytical techniques like centrifugatin, spectrophotometry, pH meter, Chromatography, Electrophoresis, PCR and ELISA
  4. Use Computational tools in the field of Biology
9.00

Microscopy

 Magnification and Resolving Power

Principle and Application of Microscopy

  • Light (bright-field, dark-field and phase contrast)
  • Electron (SEM and TEM)
  • Fluorescence Microscope
12.00

Principles and use of analytical instruments:

  • Centrifugation (Density and Differential)
  •  Spectrophotometer (UV- Visible)
  • Chromatography (Paper and TLC)
  • Electrophoresis (Agarose and PAGE)
  • PCR

ELISA

9.00

The Internet and the Biologists

The Gene bank sequence database

Structure data base

Sequence analysis using GCG

10.00

Information retrieval from Biological database

NCBI data  model

Sequence Alignment and database searching Practical aspects of multiple sequence alignment

Phylogenetic analysis

5.00

Computer Basics

MS-Office: MS. Word, Power Point and Excel

Hardware and Software

Multimedia

Network Concepts(LAN,WAN)

Internet

Web Servers, Web Access

Essential Readings: 

 

  • Wilson and Wlaker. Practical Biochemistry. Cambridge, 2000.
  • Cooper. The Cell-A Molecular Approach. ASM, 1997
  • Sharma, Munjal and Shankar. A text book of Bioinformatics. Rastogi Publications, Meerut, UP.
  • Essential Bioinformatics, Jin Xiong, John Wiley and Sons. 2006.
References: 

Suggested readings:

  • Introduction to instrumental analysis-Robert Braun-McGraw Hill.
  • A biologist Guide to principles and Techniques of Practical Biochemistry-K, Wilson and K.H. GouldingElBSEdn.
  • Essentials of Biophysics, P Narayanan, New Age Int. Pub. New Delhi. 2000.
  •  Clark &Swizer. Experimental Biochemistry. Freeman, 2000.
  • Principles and Practice of Bioanalysis, R F Venn, Taylor and Francis, 2003.
  • Locquin and Langeron. Handbook of Microscopy. Butterwaths, 1983
  •  Boyer. Modern Experimental Biochemistry. Benjamin, 1993
  • Freifelder. Physical Biochemistry. Freeman, 1982.
  • John R.W. Masters. Animal Cell culture- A practical approach. IRL Press.
  •  Robert Braun. Introduction to instrumental analysis. McGraw Hill
  • David W. Mount's "Bioinformatics" [Cold Spring Harbor Press; ISBN 0879697121].
  • James Tisdall. Beginning Perl for Bioinformatics An Introduction to Perl for Biologists. Publisher: O'Reilly Media. October 2001.

 

Academic Year: 
2019-2020
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