Tools and Techniques

Paper Code: 
LSC-143 D
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
12.00
Unit I: 
UNIT I

Spectroscopy: Spectroscopy and Spectrometry, Types of Spectroscopy. Atomic Spectroscopy: Flame Emission Spectroscopy and X- Ray Spectroscopy Molecular Spectroscopy: IR Spectroscopy, UV Spectroscopy and Visible Spectroscopy Nuclear Spectroscopy: NMR Spectroscopy

12.00
Unit II: 
UNIT II

Microscopy: Magnifiers and microscopes, simple and compound microscopes. Types of microscopy – Optical Microscopy (Bright Field and Dark Field Microscopy, Phase contrast Microscopy, Fluorescence Microscopy, Confocal and Wide Field Deconvolution Microscopy ); Electron Microscopy (SEM, TEM).

12.00
Unit III: 
UNIT III

Histology of Tissues: Selection of material, chemical fixation, fixatives, tissue dehydration using a graded solvent series of ethanol or acetone, rehydration, infiltration and embedding tissues, sectioning, mounting and staining, alternate methods of microtomy (cryotome, cryostat and vibratome)

12.00
Unit IV: 
UNIT IV

Chromatography: Technique, types, applications and limitations.

Techniques based on bed shape- Column Chromatography, Paper Chromatography and Thin Layer Chromatography
Techniques based on physical state of mobile phase- Gas Chromatography, Liquid Chromatography and High Pressure Liquid Chromatography (HPLC). Techniques based on separating mechanism- Ion Exchange Chromatography and Size Exclusion Chromatography
12.00
Unit V: 
UNIT V

Microbiological techniques: Sterilization and disinfection, Simple and Complex Media, Preparation of media -solid and liquid media, nutrient agar slabs, slants and plates, Staining techniques, simple and differential staining, Instrumentation, Inoculation, Pure culture techniques: streak plate, pour plate and spread plate method.

Essential Readings: 

A Biology Guide to Principles and Techniques of Practical Biochemistry. .Wilson, K & Goulding, KH. ELBS edition. Wilson, K & Goulding, KH. ELBS edition.

• Introduction to Instrumental Analysis. Robert Brown. Mc Graw Hill 

• Introduction to Practical Molecular Biol Introduction to Practical Molecular Biology. Dabre, PG. John Wiley & Sons ogy. Dabre, PG. John Wiley & Sons Ltd. Ltd.

• Kuby Immunology (fourth edition). Golds, RA. Thomas J. Kintz, Barbara,  A. Osborne, Freeman & Co., New York.A. Osborne, Freeman & Co., New York.

• Microbiological Applications: A Laboratory Manual in General Microbiology. WCG; WnC Brown Publish Benson, HJ. WCG; WnC Brown Publishers.ers.ers.ers.

• Microbiology, A Laboratory Manual. Cappuccino, JG and Sherman, N. .Addison Wesley.

• Molecular and Cellular Methods in Biology & Medicine. 1995. Win, K, Kim T. Cold Spring Harbor Laboratory Press, New York. aniatis,

Academic Year: 
2023-2024
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