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IIS University

Admissions

It is a University which is committed to excellence and overall development of the student and one where talent is nurtured and honed in the best possible manner. Come, be a part of our never-ending journey of accomplishments and accolades.

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Admissions

It is a University which is committed to excellence and overall development of the student and one where talent is nurtured and honed in the best possible manner. Come, be a part of our never-ending journey of accomplishments and accolades.

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Placements

Excellent education not only imparts knowledge, but also paves the way for a promising and successful career. The Placement Cell at The IIS University has numerous success stories to narrate in this regard...

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Life@IIS Univ

Life @ IISU is nothing less than a kaleidoscope of myriad hues; complete with vibrant events and memorable occasions to cherish for a lifetime.

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Academics

Taking the age-old tradition of enlightenment through education forward, The IIS University - in its continuous pursuit of excellence - firmly believes that an institution’s academic profile determines its worth and value. Therefore, on offer are a wide variety of both conventional and contemporary programmes of study.

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Research

To stimulate curiosity and cater to the global needs of a dynamic education, The IIS University - since inception - has been promoting and encouraging research-based experiential learning among all its students.

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About Us

The IIS University, with its perfect amalgamation of innovation, inspiration and synergy, aims to be a model institution for students across disciplines and programmes.

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IIS Univ
  • Admissions

    It is a University which is committed to excellence and overall development of the student...

  • Placements

    Excellent education not only imparts knowledge, but also paves the way for...

  • Life@IIS Univ

    Life @ IISU is nothing less than a kaleidoscope of myriad hues; complete with...

  • Academics

    Taking the age-old tradition of enlightenment through education forward,...

  • Research

    To stimulate curiosity and cater to the global needs of a dynamic education,...

  • About Us

    The IIS University, with its perfect amalgamation of innovation, inspiration...

IIS (Deemed to be University)

Gurukul Marg, SFS, Mansarovar, Jaipur 302020, (Raj.) India

Phone:- +91-141-2400160-61, 2397906-07,

Fax: 2395494,2781158, Toll Free No.: 1800 180 7750

Tools and Techniques | Zoology

Tools and Techniques

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Paper Code: 
LSC 143 D
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
12.00
Unit I: 
UNIT I

Spectroscopy: Spectroscopy and Spectrometry, Types of Spectroscopy. Atomic Spectroscopy: Flame Emission Spectroscopy and X- Ray Spectroscopy Molecular Spectroscopy: IR Spectroscopy, UV Spectroscopy and Visible Spectroscopy Nuclear Spectroscopy: NMR Spectroscopy

12.00
Unit II: 
UNIT II

Microscopy: Magnifiers and microscopes, simple and compound microscopes. Types of microscopy – Optical Microscopy (Bright Field and Dark Field Microscopy, Phase contrast Microscopy, Fluorescence Microscopy, Confocal and Wide Field Deconvolution Microscopy ); Electron Microscopy (SEM, TEM).

12.00
Unit III: 
UNIT III

Histology of Tissues: Selection of material, chemical fixation, fixatives, tissue dehydration using a graded solvent series of ethanol or acetone, rehydration, infiltration and embedding tissues, sectioning, mounting and staining, alternate methods of microtomy (cryotome, cryostat and vibratome).

12.00
Unit IV: 
UNIT IV
Chromatography: Technique, types, applications and limitations.
Techniques based on bed shape- Column Chromatography, Paper Chromatography and Thin Layer Chromatography
Techniques based on physical state of mobile phase- Gas Chromatography, Liquid Chromatography and High Pressure Liquid Chromatography (HPLC). Techniques based on separating mechanism- Ion Exchange Chromatography and Size Exclusion Chromatography
12.00
Unit V: 
UNIT V

Microbiological techniques: Sterilization and disinfection, Simple and Complex Media, Preparation of media -solid and liquid media, nutrient agar slabs, slants and plates, Staining techniques, simple and differential staining, Instrumentation, Inoculation, Pure culture techniques: streak plate, pour plate and spread plate method.

References: 
Books Recommended:
 
• A Biology Guide to Principles and Techniques of Practical Biochemistry. .Wilson, K & Goulding, KH. ELBS edition. Wilson, K & Goulding, KH. ELBS edition.
• Introduction to Instrumental Analysis. Robert Brown. Mc Graw Hill 
• Introduction to Practical Molecular Biol Introduction to Practical Molecular Biology. Dabre, PG. John Wiley & Sons ogy. Dabre, PG. John Wiley & Sons Ltd. Ltd.
• Kuby Immunology (fourth edition). Golds, RA. Thomas J. Kintz, Barbara,  A. Osborne, Freeman & Co., New York.A. Osborne, Freeman & Co., New York.
• Microbiological Applications: A Laboratory Manual in General Microbiology. WCG; WnC Brown Publish Benson, HJ. WCG; WnC Brown Publishers.ers.ers.ers.
• Microbiology, A Laboratory Manual. Cappuccino, JG and Sherman, N. .Addison Wesley.
• Molecular and Cellular Methods in Biology & Medicine. 1995. Win, K, Kim T. Cold Spring Harbor Laboratory Press, New York. aniatis,
Academic Year: 
2019-2020
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