SPECIAL PAPER CELL AND MOLECULAR BIOLOGY II

Paper Code: 
ZOL 423 B
Credits: 
4
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course objectives

This course will enable the students to -

  1. Get an insight into the ability of our immune system to defend against invading pathogens in a logical fashion.
  2. Distinguishes between innate and acquired immunity; Humoral and cell mediated immunity and the consequences of its failure like autoimmunity, hypersensitivity and immunodeficiencies.
  3. Know about the role of effector cells and molecules of the immune system as complements and antibodies.

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL 423 (B)

Cell And Molecular Biology II

Students should be able to:

CO100: Conceptualize how the innate and adaptive immune responses coordinate to fight invading pathogens.

CO101: Determine what immunomodulatory strategies can be used to enhance immune responses or to suppress unwanted immune responses such as might be required in hypersensitivity reactions, transplantations or autoimmune diseases.

CO102: Critically review the structure of antigen and antibody and their specific interactions.

CO103: Explore strategies to improve existing vaccines and how to approach these.

Approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Field practical

 

Class test

Semester end examinations,

Quiz

Assignments,

Presentations

Surprise test

Open book test

 

12.00
Unit I: 
UNIT I

Molecular Immunology.

  •     Innate (nonspecific) immunity.
  •      Adaptive (specific) Immunity.

Cells and organs of immune system.Hematopoiesis

  •     B-lymphocytes, T-lymphocytes, Null cells.
  •     Mononuclear cells
  •     Granulocytic cells     
  • Mast Cells.
  •     Dendritic cells.

Organs of immune system.

Lymphatic system.

  •     Primary lymphoid organs
  •     Secondary    lymphoid    organs  

Antigens.

  •     Immunogenicity versus antigenicity.
  •     Factors  influence immnogenicity
  •     Contribution   of  Biological   system. (Genotype   of  therecipient    animal,    immunogen    dosage    and    route    ofadministration, adjuvants).
  •     Epitopes
  •     Haptans.
12.00
Unit II: 
UNIT II

Immunoglobulins : Structure and function.

  •     Molecular structure of Ig, Light chain and Heavy chain
  •     Immunoglobulin domains.
  •     Immunoglobulin classes
  •     Immunoglobulin-mediated     effector    functions                                     
  •     Antigenic determinants on immunoglobulin (isotype, allotype and idiotype).

Generation of antibody diversity.

  •     Multiple germ line V, D and J gene segments.
  •     Combinatorial V-J and V-D-J joining.
  •     Junctional flexibility.
  •     P-addition and N-addition.
  •     Association of heavy and light chain.

Class switching among constant region genes.

  •     Expression of Ig genes.
  •     Regulation of Ig gene transcription.
  •     Antibody genes and antibody engineering.
12.00
Unit III: 
UNIT III

Antigen-Antibody Interaction.( in vivo and in vitro)

Major Histocompatibility complex.

  •     General organization and inheritance of MHC.
  •     Genomic maps of MHC genes.
  •     MHC molecules and genes.
  •     Cellular distribution of MHC molecules.
  •     MHC and disease susceptibility.

Mechanism of Humoral and cell mediated immunity

12.00
Unit IV: 
UNIT IV

Antigen Processing and Presentation.

  •     Role of antigen presenting cell.
  •     The cytosolic pathways.Endogenous antigens :
  •     The endocytic pathway Exogenous antigens
12.00
Unit V: 
UNIT V

Vaccine.

  •     Active and passive immunization
  •     Types of vaccines

AIDS and other immunodeficiencies.

Autoimmunity: Proposed mechanisms for induction of autoimmunity

  •     Systemic autoimmune diseases
  •     Organ specific autoimmune disease
Essential Readings: 

Essential readings

    Karp  Cell and Molecular Biology. Concepts and experiments. John Wiley and sons. Inc. New York, 1999

    Malacinski G.M. and Frifeilder D. Essentials of Molecular Biology Jones and Bartlett Publishers Boston, 1999

    Cooper, G .M. The cell A molecular approach. ASM Press, Washington DC, 2000.

    Shelve P. and Blanch DEW, Cell and Molecular Biology. John Wile) and Sons Inc., New York, 1994.

    Darnell, J.L. Lodish, H and Baltimore, D. Molecular Cell Biology, Scientific American Books Inc. New York.

    Watson, J.D., Hopkins, N.H. Roberts, J.W Steitz, J.A. and Weiner A.M. Molecular Biology of the Gene. The Benjamin/Cumings Pub. Co. Inc. California

    Gardner, EJ. Simons, MJ. andSnustad, D.P. Principles of Genetics. John Wiley .& Sons Inc. New York

    Voet, D. and Voet, J.G. Biochemistry, John Wiley & Sons Inc New York.

    Zubay, G.L. Parson, WW and Vance, D.E. Principles of Biochemistry. Wm.C. Brown Publishers, Oxford, England.

    Goldsby, RA..Kindt, T.J. and Osborne, B.A. Kuby Immunology, WH Free- I man & Co. New York.

Suggested reading:

    Alberts B, Bray D, LewioJ, RaffM, Roberts K and Watson J.D. Molecular Biology of the Cell. Garland Publishing, Inc. New York, 1994.

    Glick B. R. and Pasternak 1.1. Molecular Biotechnology Principles and Applications of Recombinant DNA. ASM Press, Washington, 1998.

    Bolrover S.R. Hyams J.S., Jones S. Shephard E.A. and White H.A. From genes to cells. Wiley-liss, New York, 1997.

    Winnacker E.L. From genes to clones Introduction to gene technology Panima Education BookAgency, New Delhi. æ VCH Publishers, New York, 1987.

    Giese A. C. Cell Physiology, WB. Saunders Co., Philadelphia, 1979

    Freifelder D. Molecular Biology, Naroja Publishing House, New Delhi, 1997.

    Frieerg E.C., Walker G.C. and Siede, W DNA Repair and Mutagenesis. ASM Press Wadbington DC, 1995.

    Abbas. AK Lichtman, AR. and Pahes, J.S. Molecular Immunology, WB Saunders & Co, London

    Cruse. J.M. and Lewis, RE. Atlas of Immunology, CRC Press,New York.

    Twyman R. M. and Wisden W. Advanced Molecular Biology A Concise Reference Viva Books Pvt. Ltd. New Delhi: 1999

    Meyers R.A. Molecular Biology and Biotechnology A comprehen- sive desk Reference. VCH Publishers, Inc. New York, 1995

    Lodish H, BerkA, Zipurjky S. L., Matsudaira P, Baltimore D. and Darnell 3. Molecular Cell Biology W.H. freeman and company New York, 2000.

Academic Year: 
2020-2021
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