PRACTICAL SPECIAL PAPER HUMAN GENETICS I

Paper Code: 
ZOL 324 D
Credits: 
8
Contact Hours: 
240.00
Max. Marks: 
100.00
Objective: 

Course objectives

This course will enable the students to -

  1. Hands on experience of Chromosomal preparation and karyotyping
  2. Culture of Drosophila and identification of normal and mutant flies
  3. To get practical knowledge of DNA isolation, purification and quantification
  4. To get familiar with restriction endonucleases and electrophoresis

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

ZOL324 D

 

Human genetics I

(Practical)

 

Students will be able to

CO80: Perform Chromosomal preparation and karyotyping

CO81: Culture Drosophila in laboratory and identify normal and mutant flies

CO82:  Isolate, purify and quantify DNA, use of Restriction endonucleases and Electrophoresis.

Approach in teaching:

Practical, Reading assignments, Demonstration

 

Learning activities for the students:

Practical  knowledge, Effective questions, Seminar presentation

 

Class test

Semester end examinations

Viva voce

 

 

 
  •     Identification of normal and mutant flies ( Drosophila melanogaster)
  •     Preparation of Drosophila polytene Chromosomes (temporary and permanent preparations)
  •     Drosophila genetic crosses
  •     Chromosome preparation from mouse bone marrow and human blood lymphocytes
  •     G-banding
  •     Karyotyping
  •     Identification of inactivated X chromosome as Barr body and drumstick
  •     Genomic DNA isolation and purification from the nuclei and quantitation of DNA by spectrophotometer
  •     EcoRI digestion of total DNA and separation of fragments by  agarose gel Electrophoresis

 

Academic Year: 
2020-2021
  • Printer-friendly version
  • PDF version