PRACTICAL MOLECULAR BIOLOGY

Paper Code: 
CZOL514 P
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

Course 

Learning outcomes 

(at course level)

Learning and  teaching strategies

Assessment  Strategies 

Paper  

Code

Paper Title

CZOL514 P

PRACTICAL MOLECULAR BIOLOGY 

The students will:

CO1 : Get hands-on experience of various molecular biology techniques including gel electrophoresis and chromatography and would learn about their applications in the research field.

 

CO 2: Enhance their knowledge about wild and mutant strains of Drosophila and their important structures

 

The approach in  teaching: 

Interactive Lectures,  Discussion, Tutorials,  Reading assignments,  Demonstration, Team  teaching 

Learning activities for  the students: 

Self-learning  

assignments, Effective  questions, Simulation,  Seminar presentation,  Giving tasks, Chart  preparation

Assessment  Strategies 

Class test,  Semester end  examinations,  Quiz, Solving  problems in  tutorials,  

Assignments,  Presentation. 

 

1. Study of Polytene chromosomes from Chironomous / Drosophila larvae

2. Preparation of liquid culture medium (LB) and raise culture of E. coli

3. Estimation of the growth kinetics of E. coli by turbidity method

4. Preparation of solid culture medium (LB) and growth of E. coli by spreading and streaking

5. Demonstration of antibiotic sensitivity/resistance of E. coli to antibiotic pressure and interpretation of results

6. Quantitative estimation of salmon sperm/calf thymus DNA using colorimeter (Diphenylamine reagent) or spectrophotometer (A260 measurement)

7. Quantitative estimation of RNA using Orcinol reaction

8. Study and interpretation of electron micrographs/ photograph showing

(a) DNA replication

(b) Transcription

(c) Split genes

 
Essential Readings: 

ESSENTIAL READINGS:

● Practical Zoology, vertebrates, Lal, S.S. Rastogi Publications, Meerut

● Introductory practical Biochemistry, Sawhney S.K., Singh R. 2009. 7th edition, Narosa

publishing house Pvt. Ltd.

● Jordon, E.L., Verma P.S. Invertebrate Zoology, S Chand and Co.

● Laboratory manual in Invertebrate & Experimental Zoology, Jacob Vyas Ramesh Book

Depot, New Delhi.

● Practical Exercises in Cytology, Genetics, Plant Breeding and Biostatistics. Raghuvanshi.

 

SUGGESTED READINGS:

● Zubay G. Biochemistry, William C. Brown Pub.

● Standard methods of biochemical analysis. Thimmaiah, S.R. Kalyani Publishers, Noida

 

e-RESOURCES:

 

● Biochemistry virtual lab- Amrita- https://vlab.amrita.edu/?sub=3&brch=64

https://vlab.amrita.edu/?sub=3&brch=64&sim=1090&cnt=2

● Amrita Virtual Labs- http://biotech01.vlabs.ac.in/List%20of%20experiments.html:

 
Academic Year: