1. Study of Polytene chromosomes from Chironomous / Drosophila larvae
2. Preparation of liquid culture medium (LB) and raise culture of E. coli
3. Estimation of the growth kinetics of E. coli by turbidity method
4. Preparation of solid culture medium (LB) and growth of E. coli by spreading and streaking
5. Demonstration of antibiotic sensitivity/resistance of E. coli to antibiotic pressure and interpretation of results
6. Quantitative estimation of salmon sperm/calf thymus DNA using colorimeter (Diphenylamine reagent) or spectrophotometer (A260 measurement)
7. Quantitative estimation of RNA using Orcinol reaction
8. Study and interpretation of electron micrographs/ photograph showing
(a) DNA replication
(b) Transcription
(c) Split genes
ESSENTIAL READINGS:
● Practical Zoology, vertebrates, Lal, S.S. Rastogi Publications, Meerut
● Introductory practical Biochemistry, Sawhney S.K., Singh R. 2009. 7th edition, Narosa
publishing house Pvt. Ltd.
● Jordon, E.L., Verma P.S. Invertebrate Zoology, S Chand and Co.
● Laboratory manual in Invertebrate & Experimental Zoology, Jacob Vyas Ramesh Book
Depot, New Delhi.
● Practical Exercises in Cytology, Genetics, Plant Breeding and Biostatistics. Raghuvanshi.
SUGGESTED READINGS:
● Zubay G. Biochemistry, William C. Brown Pub.
● Standard methods of biochemical analysis. Thimmaiah, S.R. Kalyani Publishers, Noida
e-RESOURCES:
● Biochemistry virtual lab- Amrita- https://vlab.amrita.edu/?sub=3&brch=64
https://vlab.amrita.edu/?sub=3&brch=64&sim=1090&cnt=2
● Amrita Virtual Labs- http://biotech01.vlabs.ac.in/List%20of%20experiments.html: