PRACTICAL ANIMAL BIOTECHNOLOGY

Paper Code:

DZOL 611B P

Credits:

Contact Hours:

60.00

Max. Marks:

100.00

Genomic DNA isolation from E. coli
Plasmid DNA isolation (pUC 18/19) from E. coli
Restriction digestion of plasmid DNA.

Principle and working of equipment used in laboratory (Laminar Air Flow, Autoclave, Incubator, Electrophoresis chamber)
To study following techniques through photographs a. Southern Blotting b. Northern Blotting c. Western Blotting d. DNA Sequencing (Sanger's Method)
Project report on animal cell culture

Essential Readings:

ESSENTIAL READINGS:

Brown, T.A. (1998). Molecular Biology Labfax II: Gene Cloning and DNA Analysis. II Edition, Academic Press, California, USA.

• Elementary biotechnology, Gupta, P.K; Rastogi Publications; Meerut.

● R.C. Dubey, A TextBook of Biotechnology, S. Chand and Company, New Delhi. Principles of gene manipulation; An introduction to genetic engineering; Old, R.W and Primrose, S.B; Blackwell Science.

● H.D.Kumar, Molecular Biology and Biotechnology, Vikas Publishing House Pvt. Ltd. New Delhi.

● Roitt’s essential Immunology, P. Delves, et al., 11th ed., Blackwell Publishing, 2006

● Kindt, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006). Immunology, VI Edition. W.H. Freeman and Company.

SUGGESTED READINGS:

Glick, B.R. and Pasternak, J.J. (2009). Molecular Biotechnology - Principles and Applications of Recombinant DNA. IV Edition, ASM press, Washington, USA.
Griffiths, A.J.F., J.H. Miller, Suzuki, D.T., Lewontin, R.C. and Gelbart, W.M. (2009). An Introduction to Genetic Analysis. IX Edition. Freeman and Co., N.Y., USA.
Watson, J.D., Myers, R.M., Caudy, A. and Witkowski, J.K. (2007). Recombinant DNA- Genes and Genomes- A Short Course. III Edition, Freeman and Co., N.Y., USA.

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