Practical

Paper Code: 
ZOL 503
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

 

Course Objectives: 

This course will enable the students to

  1. Understand principle and working of various instruments in biotechnology laboratory
  2. Prepare culture media for microbes
  3. Culture bacteria from various samples like soil, food, water etc
  4. Perform ELISA and ouchterlony double diffusion
  5. Analyse blood group antigens on RBCs

      Course Outcomes (COs):

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Paper Code

Paper Title

 

 

 

 

 

 

ZOL 503

 

 

 

 

 

 

Practical

The students will-

CO59: Correlate the  principles  and working of various instruments in biotechnology laboratory

CO60:  Prepare  the culture media for microbial growth

CO61: Culture and isolate the  bacteria from various samples like soil, food, water etc

CO62: Analyze blood group antigens based on coagulation and perform ELISA, Ouchterlony double diffusion.

The approach in teaching: Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration,

Learning activities for the students: Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Practical skills and laboratory reports

Lab demonstration, Class test, Semester end examinations, Quiz, Solving problems in tutorials, Assignments, Presentation, Individual and group projects, Viva voce

 

   

 Biotechnology and Microbiology:

  • Principle and working of equipments used in biotechnology laboratory(SDS-PAGE apparatus, horizontal DNA Electrophoresis chamber, Laminar Airflow, Autoclave, Incubator, pH meter, Homogenizer, Ascending and Descending Chromatographic chamber, Thin Layer Chromatographic Apparatus, Microtome)
  • Preparation and use culture media for microbes
  • Study of microbes in food material(curd, milk, rotten fruits and vegetables)
  • Educational tour to any Microbiology lab (Food processing factory, Distillery, Dairy etc.)Candidates will have to submit a report on it.
  • Culturing of Bacteria from soil/water samples.

 Immunology:

  • Experiments on Antigen and Antibody reaction.
  • Blood typing
  • ELISA
  • Ouchterlony double diffusion

 

Essential Readings: 

 

   BOOKS RECOMMENDED:

●       Practical Biochemistry: Principles & Techniques; Keith Wilson& John Walker; Cambridge University Press.

●       Microbiology, A laboratory manual by Cappuccino, James,G.

●       Lab Manual of microbiology & biotechnology by K. R. Aneja. Scientific international private limited.

 

Academic Year: 
2021-2022
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