PRACTICAL

Paper Code: 
ZOL-215
Credits: 
3
Contact Hours: 
45.00
Max. Marks: 
100.00
Biochemistry II
Colorimetric/Spectrophotometric estimation of :
Carbohydrate-Glucose, Glycogen
Protein
Nucleic acids: DNA,RNA
Study of temperature effect on enzyme action: Salivary amylase 
 
 
Animal Diversity III
Study of museum specimens and slides:
 
Phylum  Annelida-Nereis, Heteronereis-parapodia, Earthworm, Leech
Dissection: Earthworm- Anatomy, Digestive, Nervous System
Permanent mount: Septal nephridia, Ovary,Nerve ring.
 
Phylum Arthropoda: Daphnia, Cyclops, Scorpion, KingCrab, Crab, Eupagurus, Lepas, Balanus, Millipede, Termite, Centipede, Praying-mantis, Peripatus
Dissection: Prawn-General anatomy, Appendages, Nervous &Digestive system
Permanent mount: Hastate plate, Statocyst
 
Phylum-Mollusca:  Chiton, Unio, Dentalium, Nautilus, Loligo, Octopus, Sepia
 
Larval forms- Miracidium, Sporocyst, Redia, Cercaria, Cysticercus larva, Nauplius,Zoea,Mysis,cypris,Megalopa.
Phylum Echinodermata: Echinus, Starfish, Cucumaria, Antedon
 
Molecular Biology:
 
DNA isolation 
Biochemical estimation of DNA content
Electrophoretic Separation proteins from serum
 
Genetics & Biostatitics
Study of Drosophila life cycle & idea about its culture
Identification of Male & Female Drosophila
 
Identification of Wild & mutant (Yellow body, Ebony, Vestigial wings, White eye) of  Drosophila
Study of permanent prepared slide - Sex comb, Salivary gland chromosomes
Identification of Blood groups(A,B, O & Rh factor)
 
Preparation of frequency tables and graphs manually and with help of computers.
Calculation of Mean, Median, Mode.
Calculation of standard deviation, variance and standard error of the mean.
Chi square test for independence of attributes, t test 
 
 
Essential Readings: 
A Biologist's Guide to Principles and Techniques of Practical Biochemistry, K.Wilson & KH Goulding, ELBS Edition 1986. 
Invertebrate Zoology Barnes, RD. W.B.Saunders Co., Philadelphia 
A Biology of higher invertebrates, Russel-Hunter, WD. McMillan Co. Ltd., London 
 
Academic Year: