Practical

General physiology

• Comparison of RBC and WBC in different groups of Vertebrates.
• Photometric determination of haemoglobin in blood sample.
• Determination of MCV,MCH,MCHC of the given sample of blood
• Demonstration of the following in blood:clotting time,erythrocyte sedimentation rate,haemolysis and crenation
• Determination of Haematocrit value of blood sample.

Tools and Techniques

• Microscopes
• Surgical techniques Adrenalectomy,Ovarectomy,Castration
• Standardisation of oculometer and measurements of tubular diameter,cell heights.
• To study the structure & function of Centrifuge.
• To study the principle of UV & visible light spectroscopy.
• To study the principle of cyclomixer.
• Flow cytometry
• To observe the sub cellular fractionation of rat liver.
• Measurement of diameter of thyroid follicles.
• Alizarin preparation.

Molecular Biology & Biotechnology

• Chromosomal counting in bone marrow cells of a mammal
• Isolation of RNA
• Restriction  digestion of DNA
• Agarose gel electrophoresis of DNA
• Separation of amino acids on paper & thin layer chromatography
• Separation of protein on SDS PAGE

Environmental Science and Wildlife Management

Water Evaluation Analysis( minimum of five analysis methods)

• Determination of chloride the given water sample.
• Estimation of total hardness of given water sample.
• To determine the acidity of water.
• To determine the alkalinity of water.
• To estimate total solid in water.
•   To estimate total dissolved solid in water sample.
•   To determine the dissolved oxygen in given water sample.
•   To determine the BOD of given water sample. 
• To determine the free CO₂ of given water sample.
•   To estimate the Calcium hardness of water.
•   To estimate Magnesium hardness of given water sample.

Soil Analysis( minimum of five analysis methods)

Ø  Determination of organic matter in soil sample.

Ø  Determination of Co₃- & HCo₃- in soil sample.

Ø  Estimation of chlorophyll in plant material.

Ø  Determination of leaf area index.

Ø  Determination of residual chloride in water sample.

Ø  Determination of moisture in soil.

Ø  Determination of minimum size of quadrant.

Ø  Determination of quadrate in field.

Ø  Study of community of quadrate.

Ø  Determination of dust holding capacity of leaves.

Ø  Determination of bulk density of the given soil sample.

Ø  Determination of water holding capacity of soil.

Ø  Test for presence of inorganic salts in soil.

A visit to a sanctuary/National Park./Educational Tour (Preparation of Report)