PRACTICAL

Animal Diversity I

Study of museum specimens and slides:

·        Protozoa :Paramecium(WM), Binary fission, Conjugation, Entamoeba, Trypanosoma, Plasmodium, Monocystis, Opalina, Vorticella, Balantidium, Nyctotherus, Euglena

·        Porifera: Sycon L.S & T.S, Sponge spicules, Spongin fibres

     Leucosolenia, Euplectella, Spongilla.

·        Coelentrata: Hydra, Obelia(polyp), Aurelia, Sea anemone, Gorgonia, Physalia, Vellela, Alcyonium, Millepora, Pennatula

o   Platyhelminthes: Planaria, Fasciola,Taenia-- Scolex, Gravid proglottid

·        Aschelminthes: Ascaris(Male & Female), Dracunculus

Cell Biology

Principle & working of

·        Dissecting microscope

·        Compound microscope   

Fixation and staining

·        Fixatives:- Carnoys, Bouins, formalin

·        Stains:- Acetocarmine, Acetorcein, Eosin,        Haematoxylin

·        Double & Single staining

·        Staining of Euglena,Hydra,Obelia,Foraminiferous shell,Sponge spicules,Sponge gemmules,Spongin fibres

                 Principle & working of Centrifuge (Only Demonstration)

                 Mitosis- Onion root tip

                 Meiosis- Grasshopper testis