Practical

Paper Code: 
ZOL-103
Credits: 
2
Contact Hours: 
60.00
Max. Marks: 
100.00
Objective: 

COURSE OBJECTIVES

Study of laboratory specimens based on their external features and appearance of various phyla.
To gain knowledge about various fixation and staining techniques using different biological specimens.
To enhance their knowledge about different mitotic stages by preparing temporary slides of the onion root tip and visualizing the stages by their key features.
 

Learning outcome (at course level)

Learning and teaching strategies

Assessment Strategies

CLO-11 Enhance knowledge about animal kingdom by studying representative animals from each phylum.

CLO-12 To identify and describe different laboratory specimens based on their classification and characters.

CLO-13 Students will get hands-on experience of staining techniques using different biological samples.

CLO-14 Acquire knowledge about different mitotic stages by preparing temporary slides of the onion root tip and visualizing the stages identifying their characteristics.

 

Approach in teaching:

Substantial laboratory based practical demonstration,

Discussion, Action Research, Project, Field Trip

 

Learning activities for the students:

Laboratory practices,

Field activities, Simulation, Presentation, Giving tasks       

●Technology enabled learning observation

●Class test

●Semester end examinations

●Quiz

●Assignments

●Presentation

●Report writing

 
Unit I: 
Animal Diversity I
Study of museum specimens and slides:
Protozoa :Paramecium(WM), Binary fission, Conjugation, Entamoeba, Trypanosoma, Plasmodium, Monocystis, Opalina, Vorticella, Balantidium, Nyctotherus, Euglena
Porifera: Sycon L.S & T.S, Sponge spicules, Sponginfibres
Leucosolenia, Euplectella, Spongilla.
Coelentrata: Hydra, Obelia(polyp), Aurelia, Sea anemone, Gorgonia, Physalia, Vellela, Alcyonium, Millepora, Pennatula
Platyhelminthes: Planaria, Fasciola,Taenia-- Scolex, Gravid proglottid
Aschelminthes:Ascaris(Male & Female), Dracunculus
 
Unit II: 
Cell Biology
Principle & working of
Dissecting microscope
Compound microscope    
Fixation and staining
Fixatives:- Carnoys, Bouins, formalin
Stains:- Acetocarmine, Acetorcein, Eosin,        Haematoxylin
Double & Single staining 
Staining of Euglena,Hydra,Obelia,Foraminiferous shell,Sponge spicules,Sponge gemmules,Spongin fibres
 
 Principle & working of Centrifuge (Only Demonstration)
 
 Mitosis- Onion root tip (Temporary slide Preparation)
 
Meiosis-Study of Permanent slides
Essential Readings: 
Pechenik, J.A. Biology of invertebrates,TataMc-Graw Hill Ltd, New Delhi.
Jordon, E.L., Verma P.S. Invertebrate Zoology, S Chand and Co.
Practical Zoology, Invertebrates, Lal, S.S. Rastogi Publications, Meerut
Practical Zoology, Invertebrates, Kohli,K.S.,Ansari, A.S. Ramesh Book Depot, New Delhi.
Laboratory manual in Invertebrate & Experimental Zoology, Jacob VyasRamesh Book Depot, New Delhi.
Practical Zoology, Jacob, D., Bhatia,A.L., Kohli,K.S., Vyas,D.K.Ramesh Book Depot, New Delhi
Biology of Animals. C.P. Hickmann,  L.S. Roberts and A. Larson. McGraw Hill Company, New York.
Practical Zoology, Jacob, D., Bhatia,A.L., Kohli,K.S., Vyas,D.K.Ramesh Book Depot, New Delhi.
 
Academic Year: