Practical

General physiology

Ø  Comparison of RBC and WBC in different groups of Vertebrates.

Ø  Photometric determination of haemoglobin in blood sample.

Ø  Determination of MCV,MCH,MCHC of the given sample of blood

Ø  Demonstration of the following in blood:clotting time, erythrocyte sedimentation rate, haemolysis and crenation

Ø  Determination of Haematocrit value of blood sample.

Tools and Techniques

Ø  Microscopes

Ø  Surgical techniques Adrenalectomy, Ovarectomy, Castration

Ø  Standardisation of oculometer and measurements of tubular diameter, cell heights.

Ø  Measurement of diameter of thyroid follicles.

Ø  To study the structure & function of Centrifuge.

Ø  To study the principle of UV & visible light spectroscopy.

Ø  To study the principle of cyclomixer.

Ø  Flow cytometry

Ø  To observe the sub cellular fractionation of rat liver.

Ø  Alizarin preparation.

Molecular Biology & Biotechnology

Ø  Chromosomal counting in bone marrow cells of a mammal

Ø  Isolation of RNA

Ø  Restriction  digestion of DNA

Ø  Agarose gel electrophoresis of DNA

Ø  Separation of amino acids on paper & thin layer chromatography

Ø  Separation of protein on SDS PAGE

Environmental Science and Wildlife Management

Water Evaluation Analysis (minimum of five analysis methods)

Ø  Determination of chloride the given water sample.

Ø  Estimation of total hardness of given water sample.

Ø  To determine the acidity of water.

Ø  To determine the alkalinity of water.

Ø  To estimate total solid in water.

Ø  To estimate total dissolved solid in water sample.

Ø  To determine the dissolved oxygen in given water sample.

Ø  To determine the BOD of given water sample.

Ø  To determine the free CO₂ of given water sample.

Ø  To estimate the Calcium hardness of water.

Ø  To estimate Magnesium hardness of given water sample.

Soil Analysis( minimum of five analysis methods)

Ø  Determination of organic carbon and organic matter in soil sample.

Ø  Determination of CO^3- & HCO^3- in soil sample.

Ø  Estimation of chlorophyll in plant material.

Ø  Determination of leaf area index and percent leaf injury of sample from polluted sites.

Ø  Determination of residual chloride in water sample.

Ø  Determination of moisture in soil.

Ø  Determination of frequency, density and abundance of community of quadrant

Ø  Determination of dust holding capacity of leaves.

Ø  Determination of bulk density of the given soil sample.

Ø  Determination of water holding capacity of soil.

Ø  Test for presence of inorganic salts in soil.

A visit to a sanctuary/National Park./Educational Tour (Preparation of Report)