Paper Code: 
Contact Hours: 
Max. Marks: 

Course Objectives:

This course will enable the students to

1. Understand principle and working of various instruments in biotechnology laboratory

2. Preparation of culture media for microbes

3. Culture bacteria from various samples like soil, food, water etc

4. Perform ELISA and ouchterlony double diffusion

5. Analysis of blood groups



Course Outcomes (COs):


Learning outcomes

(at course level)

Learning and teaching strategies



Paper Code

Paper Title



The students will be able to –

CO59: To understand principle and working of various instruments in biotechnology laboratory

CO60: To prepare and culture media for microbes

CO61: To culture bacteria from various samples like soil, food, water etc

CO62: To analyse blood groups and perform ELISA


The approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration,


Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Practical skills and laboratory reports

Lab demonstration

Class test

Semester end examinations


Solving problems in tutorials

Assignments Presentation

Individual and group projects

Viva voce



Unit I: 
Biotechnology and Microbiology:
Principle and working of equipments used in biotechnology laboratory(SDS-PAGE apparatus, horizontal DNA Electrophoresis chamber, Laminar Airflow, Autoclave, Incubator, pH meter, Homogenizer, Ascending and Descending Chromatographic chamber, Thin Layer Chromatographic Apparatus, Microtome)
Preparation and use culture media for microbes
Study of microbes in food material(curd, milk, rotten fruits and vegetables)
Educational tour to any Microbiology lab (Food processing factory, Distillery, Dairy etc.)Candidates will have to submit a report on it.
Culturing of Bacteria from soil/water samples.


Unit II: 
Experiments on Antigen and Antibody reaction.
Blood typing
Ouchterlony double diffusion
Essential Readings: 
Practical Biochemistry: Principles &Techniques; Keith Wilson& John Walker; Cambridge University Press.
Microbiology, A laboratory manual by Cappucino, James,G.
Lab Manual of microbiology & biotechnology by K. R. Aneja. Scientific international private limited.
Academic Year: