Practical

Paper Code: 
ZOL 115
Credits: 
4
Contact Hours: 
120.00
Max. Marks: 
100.00
Objective: 

Course objectives:

 

This course will enable the students to –

 

  1. Study of laboratory specimens based on their external features and appearance of various phyla.
  2. To gain knowledge about various fixation and staining techniques using different biological specimens.
  3. To enhance their knowledge about different mitotic stages by preparing temporary slides of the onion root tip and visualizing the stages by their key features.
  4. Learn various qualitative and quantitative biochemical techniques to differentiate between different biomolecules based on their physical and chemical properties.
  5. Analysis of the effect of temperature on the enzymatic activity and gain hands-on expertise in the separation of various solutes using chromatographic techniques.

 

Course Outcomes (COs):

Course

Learning outcomes

(at course level)

Learning and teaching strategies

Assessment

Strategies

Paper Code

Paper Title

ZOL 115

Practical

Students will be able to

CO 19: Identify and describe different laboratory specimens based on their external features and appearance and their categorical relation with various phylums.

CO 20: Get hands-on experience of various fixation and staining techniques using different biological specimens.

CO 21: Enhance their knowledge about different mitotic stages by preparing temporary slides of the onion root tip and visualizing the stages by their key features.

CO 22: Learn various qualitative and quantitative biochemical techniques to differentiate between different biomolecules based on their physical and chemical properties.

CO 23: To analyze the effect of temperature on the enzymatic activity and gain hands-on expertise in the separation of various solutes using chromatographic techniques.

The approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading assignments, Demonstration, Team teaching

 

Learning activities for the students:

Self-learning assignments, Effective questions, Simulation, Seminar presentation, Giving tasks, Demonstration by Virtual software.

 

 

Class test

Semester end examinations,

Quiz,

Assignments,

Presentation

Surprise test

Open book test

 

 

 

Animal Diversity I & II

Study of museum specimens and slides:

Protozoa :Paramecium(WM), Binary fission, Conjugation, Entamoeba, Trypanosoma, Plasmodium, Monocystis), Opalina, Vorticella, Balantidium (Rectal Ciliate, Nyctotherus(Rectal Ciliate), Euglena, Giardia, Elphidium

Porifera:Sycon L.S & T.S, Sponge spicules, Spongin fibres

Leucosolenia, Euplectella, Spongilla.

Coelenterata: Hydra, Obelia(polyp), Aurelia, Sea anemone, Gorgonia, Physalia, Vellela, Alcyonium, Millepora, Pennatula

Platyhelminthes: Planaria, Fasciola,Taenia-- Scolex, Gravid proglottid, Fasicola hepatica and its Larval Forms

Aschelminthes:Ascaris(Male & Female), Dracunculus, Wuchereria, Hook Worm

Any  one animal from Minor Phyla: Ctenophora, Rotifera, Onychophora

Cell Biology

Fixation and staining:

Fixatives:- Carnoys, Bouins, formalin

Stains:- Acetocarmine,Acetorcein, Eosin,  Haematoxylin

 

Double & Single staining :

Staining of Euglena,Hydra,Obelia,Foraminiferous shell,Sponge spicules,Sponge gemmules,Spongin fibre

   Mitosis- Onion root tip (Temporary Slide preparation)

   Meiosis- (Study of Permanent slides)

Biochemistry

  • Identification of lipids, proteins & Carbohydrates in the given sample
  • Identification of different kinds of mono, di & polysaccharides in the given sample
  • Quantitative analysis of cholesterol and glycogen using colorimeter
  • Separation of Dyes by circular paper chromatography method
  • Analysis of enzymatic activity                      
  • Liver Catalase
Essential Readings: 

 

  • Pechenik, J.A. Biology of Invertebrates,TataMc-Graw Hill Ltd, New Delhi.
  • Jordon, E.L., Verma P.S. Invertebrate Zoology, S Chand and Co.
  • Practical Zoology, Invertebrates, Lal, S.S. Rastogi Publications, Meerut
  • Practical Zoology, Invertebrates ,Kohli,K.S.,Ansari, A.S. Ramesh Book Depot, New Delhi.
  • Laboratory manual in Invertebrate & Experimental Zoology, Jacob Vyas Ramesh Book Depot, New Delhi.
  • Biology of Animals. C.P. Hickmann,  L.S. Roberts and A. Larson. McGraw Hill Company, New York.
  • Standard methods of biochemical analysis.Thimmaiah, S.R. KalyaniPublishers,Noida
  • Practical Zoology, Jacob,D. Ramesh Book Depot, Jaipur.

 

 

 

 

Academic Year: 
2020-2021
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