PRACTICAL

Paper Code: 
ZOL-115
Credits: 
4
Max. Marks: 
100.00
Objective: 

Animal Diversity I & II

Study of museum specimens and slides:

  • Protozoa :Paramecium(WM), Binary fission, Conjugation, Entamoeba, Trypanosoma, Plasmodium, Monocystis), Opalina, Vorticella, Balantidium (Rectal Ciliate, Nyctotherus(Rectal Ciliate), Euglena, Giardia, Elphidium
  • Porifera: Sycon L.S & T.S, Sponge spicules, Spongin fibres

     Leucosolenia, Euplectella, Spongilla.

  • Coelentrata: Hydra, Obelia(polyp), Aurelia, Sea anemone, Gorgonia, Physalia, Vellela, Alcyonium, Millepora, Pennatula
  • Platyhelminthes: Planaria, Fasciola,Taenia-- Scolex, Gravid proglottid,Fasicola Hepatica and its Larval Forms
    • Aschelminthes: Ascaris(Male & Female), Dracunculus, Wuchereria, Hook Worm
  • Any  one animal from Minor Phyla: Ctenophora, Rotifera, Onychophora

Cell Biology

Fixation and staining

  • Fixatives:- Carnoys, Bouins, formalin
  • Stains:- Acetocarmine, Acetorcein, Eosin,        Haematoxylin
  • Double & Single staining
  • Staining of Euglena,Hydra,Obelia,Foraminiferous shell,Sponge spicules,Sponge gemmules,Spongin fibres

                 Mitosis- Onion root tip

                 Meiosis- Grasshopper testis 

Biochemistry

  • Identification of lipids, proteins & Carbohydrates in the given sample
  • Identification of different kinds of mono, di & polysaccharides in the given sample
  • Quantitative analysis of cholesterol and glycogen using colorimeter
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  •  
  • Separation of Dyes & Amino Acid by circular paper chromatography method
  •  
  • Analysis of enzymatic activity                                     
  • Liver Catalase
  • Salivary amylase

        

 

 

Essential Readings: 
  • Practical Zoology, vertebrates, Lal, S.S. Rastogi Publications, Merrut
  • Practical Zoology, vertebrates,Kohli,K.S.,Ansari, A.S. Ramesh Book Depot, Jaipur.
  • Standard methods of biochemical analysis.Thimmaiah, S.R. Kalyani Publishers,Noida
  • Practical Zoology, Jacob,D. Ramesh Book Depot, Jaipur.