PRACTICAL

Animal Diversity I & II

Study of museum specimens and slides:

• Protozoa :Paramecium(WM), Binary fission, Conjugation, Entamoeba, Trypanosoma, Plasmodium, Monocystis), Opalina, Vorticella, Balantidium (Rectal Ciliate, Nyctotherus(Rectal Ciliate), Euglena, Giardia, Elphidium
• Porifera: Sycon L.S & T.S, Sponge spicules, Spongin fibres

     Leucosolenia, Euplectella, Spongilla.

• Coelentrata: Hydra, Obelia(polyp), Aurelia, Sea anemone, Gorgonia, Physalia, Vellela, Alcyonium, Millepora, Pennatula

• Platyhelminthes: Planaria, Fasciola,Taenia-- Scolex, Gravid proglottid,Fasicola Hepatica and its Larval Forms
  • Aschelminthes: Ascaris(Male & Female), Dracunculus, Wuchereria, Hook Worm

• Any  one animal from Minor Phyla: Ctenophora, Rotifera, Onychophora

Cell Biology

Fixation and staining

• Fixatives:- Carnoys, Bouins, formalin
• Stains:- Acetocarmine, Acetorcein, Eosin,        Haematoxylin
• Double & Single staining
• Staining of Euglena,Hydra,Obelia,Foraminiferous shell,Sponge spicules,Sponge gemmules,Spongin fibres

                 Mitosis- Onion root tip

                 Meiosis- Grasshopper testis 

Biochemistry

• Identification of lipids, proteins & Carbohydrates in the given sample
• Identification of different kinds of mono, di & polysaccharides in the given sample
• Quantitative analysis of cholesterol and glycogen using colorimeter
• Separation of Dyes by circular paper chromatography method
• Analysis of enzymatic activity                  

• Liver Catalase

• Salivary amylase