PRACTICAL

Paper Code: 
ZOL-503
Credits: 
3
Max. Marks: 
100.00
Objective: 

Biotechnology and Microbiology:

 

  • Principle and working of equipments used in biotechnology laboratory (SDS-PAGE apparatus, horizontal DNA Electrophoresis chamber, Laminar Airflow, Autoclave, Incubator, pH meter, Homogenizer, Ascending and Descending Chromatographic chamber, Thin Layer Chromatographic Apparatus, Microtome)
  • Preparation and use culture media for microbes
  • Study of microbes in food material (curd, milk, rotten fruits and vegetables)
  • Educational tour to any Microbiology lab (Food processing factory, Distillery, Dairy etc.) Candidates will have to submit a report on it.
  • Culturing of Bacteria from soil/water samples.
  • MPN Assay

 

Immunology:

 

Experiments on Antigen and Antibody reaction.

  • Blood typing
  • ELISA
  • Ouchterlony double diffusion

 

 

Essential Readings: 
  • Practical Biochemistry: Principles &Techniques; Keith Wilson& John Walker; Cambridge University Press.
  • Microbiology, A laboratory manual by Cappucino, James,G.
Academic Year: 
2017-2018
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