PRACTICAL

Biochemistry II

• Colorimetric/Spectrophotometric estimation of :

• Carbohydrate-Glucose
• Preparation of Phosphate buffer
• Isolation of protein (casein) from milk
• Study of temperature effect on enzyme action: Salivary amylase

Animal Diversity III

Study of museum specimens and slides:

Phylum  Annelida-Nereis, Heteronereis-parapodia, Earthworm, Leech

Dissection (Demonstration): Earthworm- Anatomy, Digestive, Nervous System

Permanent mount: Septal nephridia, Ovary,Nerve ring.

Phylum Arthropoda: Daphnia, Cyclops, Scorpion, KingCrab, Crab, Eupagurus, Lepas, Balanus, Millipede, Termite, Centipede, Praying-mantis, Peripatus

Dissection: (Demonstration): Prawn-General anatomy, Appendages, Nervous & Digestive system

Permanent mount: Hastate plate, Statocyst

Phylum-Mollusca:  Chiton, Unio, Dentalium, Nautilus, Loligo, Octopus, Sepia

Larval forms- Miracidium, Sporocyst, Redia, Cercaria, Cysticercus larva, Nauplius,Zoea,Mysis,cypris,Megalopa.

Phylum Echinodermata: Echinus, Starfish, Cucumaria, Antedon

Molecular Biology:

•  DNA isolation and separation by Agarose gel method
• Vertical and horizontal electrophoresis unit (demonstration only)
• Paper chromatographic separation of amino acids

Genetics & Biostatitics

• Study of Drosophila life cycle & idea about its culture
• Identification of Male & Female Drosophila
• Identification of Wild & mutant (Yellow body, Ebony, Vestigial wings, White eye) of  Drosophila
• Study of permanent prepared slide - Sex comb, Salivary gland chromosomes
• Identification of Blood groups (A,B, O & Rh factor)

• Preparation of frequency tables and graphs manually and with help of computers.
• Calculation of Mean, Median, Mode.
• Calculation of standard deviation, variance and standard error of the mean.
• Chi square test for independence of attributes, t test