PRACTICAL

Paper Code: 
25DZOL804
Credits: 
6
Contact Hours: 
12.00
Max. Marks: 
100.00
Objective: 

Course objectives:

This course will enable the students to –

1.  Design and conduct experiments, using a broad variety of experimental techniques, and interpret the data obtained to draw conclusions about whether the results do or do not support a hypothesis being tested and whether the experimental design was appropriate.

2.   Work collaboratively with other students and with faculty, understanding that most scientific knowledge/conclusions draw(s) on multiple sources of data and multiple experimental approaches.

3. Learn “best approaches” for conducting experiments including laboratory safety, data replication and quality control, record keeping, and other aspects of “responsible conduct of research”.

4. Develop problem solving skills and analytical thinking skills.

Course Outcomes: 

Course

Learning outcomes (at course level)

Learning and teaching strategies

Assessment Strategies

Course Code

Course Title

25DZOL8

04

Practical

CO155: Appraise hands-on practice

of various microscopic techniques,

occulometry, centrifugation and UV

- visible spectroscopy.

CO156: Understand the basics of

cyclomixer,        flow                        cytometry,

subcellular fractionation and Alizarin

preparation and their applications.

CO157:        Appraise       hands-on

experience    of  various  molecular

biology   techniques    including              gel

electrophoresis,                           chromatography

and      study      of           chromosomal

aberrations and would learn about

their applications in the research

field.

CO158: Analysis of water quality by

assessment of various physical and

chemical parameters and evaluate

various qualitative and quantitative

parameters of soil.

CO159:     Demonstrate  skills and

record the concepts in the form of

practical file and reflect on personal

learning         experiences                     and

experimental outcomes during viva

voce.

CO160:Contribute     effectively                               in

course-specific interaction.

Approach in

teaching:

Interactive

Lectures,

Discussion,

Tutorials,

Reading

assignments,

Demonstration,

Team teaching

Learning

activities for

the students:

Self learning

assignments,

Effective

questions,

Simulation,

Giving tasks,

Field

practical

Semester

end

examinations

Viva voce

Presentation

 

 

 

 

Tools and Techniques

Microscopes

Standardization of Occulometer and measurements of tubular diameter, cell heights. Measurement of diameter of thyroid follicles.

To study the structure & function of Centrifuge.

To study the principle of UV & visible light spectroscopy. To study the principle of cyclomixer.

Flow cytometry

To observe the subcellular fractionation of rat liver. Alizarin preparation.

Molecular Biology

Chromosomal counting in bone marrow cells of a mammal Isolation of DNA

Restriction digestion of DNA Agarose gel electrophoresis of DNA

Separation of amino acids on Course and Thin layer Chromatography

Separation of protein on SDS PAGE

Environmental Science and Wildlife Management

Water Evaluation Analysis (minimum of five analysis methods)

Determination of chloride in the given water sample.

Estimation of total hardness of given water sample. To determine the acidity of water.

To determine the alkalinity of water.

To estimate total solid in water.

To estimate the total dissolved solid in the water sample.

To determine the dissolved oxygen in a given water sample.

To determine the BOD of a given water sample.

To determine the free CO2 of a given water sample. To estimate the Calcium hardness of water.

To estimate Magnesium hardness of a given water sample.

Soil Analysis (minimum of five analysis methods)

Determination of organic carbon and organic matter in soil sample. Determination of CO3-& HCO3-in soil sample.

Estimation of chlorophyll in plant material.

Determination of leaf area index and percent leaf injury of sample from polluted sites. Determination of residual chloride in water sample.

Determination of moisture in soil.

Determination of frequency, density and abundance of community of quadrant Determination of dust holding capacity of leaves.

Determination of bulk density of the given soil sample. Determination of water holding capacity of soil.

Test for presence of inorganic salts in soil.

A visit to a sanctuary/National Park./Educational Tour (Preparation of Report)

SCHEME FOR PRACTICAL EXAMINATION:

Scheme for CA End Practical Examination

Exercise 1

Exercise II

File

Viva

Attendance

Total

 

5

 

5

 

10

 

5

 

5

 

30

Scheme for Semester End Practical Examination

Exercise I

Exercise II

Exercise III

Exercise IV

Spotting

File

Viva

Total

8

8

8

8

6x3=18

10

10

70

 

Essential Readings: 

ESSENTIAL READING:

●     Principles and Techniques in Biochemistry and Molecular Biology, Edited by Keith Wilson and John Walker, Cambridge University Press, (6th Edition),2007.

●     Handbook of methods in Environmental Studies,Vol 2 (Air, noise, soil and overburden analysis) Maiti,S.K., Jaipur Publications.

●     Handbook of Methods in Environmental Studies, Vol I, Water and Wastewater, Maiti,S.K., Jaipur Publications.

●     Fundamental Laboratory Approaches for Biochemistry and Biotechnology, A J Ninfa., D P Ballou, Fitzgerald science press, Inc.,1998.

References: 

SUGGESTED READING:

●     TextBook of Medical Biochemistry; Chatterjee, M.N and Shinde, R.; Jaypee brothers.( 2007)

●     Principles of Wildlife management; James, A. Bailey; John Wiley & sons ●

●       Introduction to Instrumental analysis; Robert Braun: Mc –Graw hill, International eds.

●       Environmental Science, Santra S.C., New Central Book Agency Pvt Ltd., Kolkata

●       Environmental Science, Cunningham andSaigo

e-CONTENT:

●     e-PGpathshala- inflibnet center

https://epgp.inflibnet.ac.in/Home/ViewSubject?ca tid=2rAs1Puvga4LW93zMe83aA ==

●     https://esj-JOURNALS:.onlinelibrary.wiley.com/journal/1438390x

●     https://bioprinciples.biosci.gatech.edu/popula tion-ecology-1/

●     https://academic.oup.com/bioscie nce/article/62/3/260/359064

●     https://www.unep.org/interactives/beat-plas tic-pollution/

●     https://epgp.inflibnet.ac.in/Home/ViewSubject?ca tid=2rAs1Puvga4LW93zMe83aA ==

●     Physiology-Swayamprabha-https://dth.ac.in/medical/course.php

●     Anatomy-SwayamPrabha- https://dth.ac.in/medical/course.php

JOURNALS:

●     American Journal of Physiology-

●     Journal of Applied Physiology

●     Frontiers in Physiology

●     Environmental Science and Pollution Research

●     Journal of Environmental Sciences

●     Bioremediation Journal

●     Archives of Environmental Contamination and Toxicology

 

Academic Year: 
2025-2026
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