Practical

Paper Code: 
ZOL-515
Credits: 
4
Contact Hours: 
120.00
Max. Marks: 
100.00
Objective: 
Course objectives:  
This course will enable the students to - 
1. Understand principle and working of various instruments in biotechnology laboratory 
2. Prepare culture media for microbes 
3. Culture bacteria from various samples like soil, food, water etc 
4. Perform ELISA and Ouchterlony double diffusion 
5. Analyze blood groups 
 
Course Outcomes (COs): 
 

Course 

Learning outcomes 

(at course level)

Learning and  teaching strategies

Assessment  Strategies 

Paper  

Code

Paper Title

ZOL-515 

Practical 

Students will  

CO 97: Design experiments based on  principle and working of various  instruments in biotechnology  laboratory 

CO 98: Formulate culture media for  microbes 

CO 99: Examine bacterial isolation  from various samples like soil, food,  water etc. 

CO100: Investigate ELISA and  Ouchterlony double diffusion 

CO 101: Analyze blood groups

The approach in  teaching: 

Interactive Lectures,  Discussion, Tutorials,  Reading assignments,  Demonstration,  

Learning activities for  the students: 

Self- learning  assignments, Effective  questions, Simulation,  Seminar presentation,  Giving tasks, Practical  skills and laboratory  reports,

Class test  

Semester end  examinations 

Viva voce

 

Biotechnology  
 
Principle and working of equipments used in biotechnology laboratory(SDS-PAGE apparatus,  horizontal DNA Electrophoresis chamber, Laminar Airflow, Autoclave, Incubator, pH meter,  Homogenizer, Ascending and Descending Chromatographic chamber, Thin Layer  Chromatographic Apparatus, Microtome) 
 
Microbiology: 
 
● Preparation and use culture media for microbes 
● Study of microbes in food material(curd, milk, rotten fruits and vegetables) 
● Educational tour to any Microbiology lab (Food processing factory, Distillery, Dairy etc.)  Candidates will have to submit a report on it. 
● Culturing of Bacteria from soil/water samples. 
● Quality testing of milk
● Disc diffusion assay 
● Spread plate and Streak Plate 
 
Immunology: 
 
Experiments on Antigen and Antibody reaction. 
● Blood typing 
● ELISA 
● Ouchterlony double diffusion
 
Essential Readings: 
● P.D. Sharma, Microbiology,Rastogi and Company, Meerut. 
● Microbiological Application,AlfredE.Brown, McGraw.Hill
● Microbiology by Totora, Pearson Education. 
References: 
SUGGESTED READINGS:
●Microbial:T.StuartWalker,W.B.Saunders Company 
● Microbiological Application,AlfredE.Brown, McGraw.Hill
● Practical Biochemistry:Principles and Techniques; Keith Wilson& John  Walker;CambridgeUniversity Press. 
● Lechiman, W.Microbiology, Glenced Pub1. Co.New York 
● Nestler, R.L. Microbiology, W.S. Saunders, Philadelphia (Student edition:Holt-Saunders  International Edition, Tokyo). 
● Salle,S.J. Fundamental Principles of Bacteriology,TataMcGraw Hill Pub. Co., New Delhi. ● Stonier, T.I. Nouderroff, and Adeloverg, E.A. General Microbiology. 
● Jacob D.IntroductoryMicrobiology,Ramesh Book Depot,Jaipur. 
● Cruick shank et al. Medical Microbiology.The English Language Book Society,London. 
 David Freifelder-Molecular Biology, Narosa Publishing House, New Delhi
 
e-RESEOURCES:
 
Academic Year: 
2022-2023
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