Practical

Paper Code: 
ZOL 503
Credits: 
2
Contact Hours: 
100.00
Max. Marks: 
60.00
Objective: 
Course Objectives:
 
This course will enable the students to 
1. Understand principle and working of various instruments in biotechnology laboratory 
2. Prepare culture media for microbes 
3. Culture bacteria from various samples like soil, food, water etc. 
4. Perform ELISA and ouchterlony double diffusion 
5. Analyse blood group antigens on RBCs 
 
 

Course

Learning outcomes 

(at course level)

Learning 

and teaching 

strategies

Assessment

Strategies

Paper 

Code

Paper Title

ZOL 

503

Practical

The students will

CO59: Correlate the principles and working of  various instruments in biotechnology laboratory 

CO60: Prepare the culture  media for microbial growth CO61: Culture and isolate the  bacteria from various samples  like soil, food, water etc

CO62: Analyse blood group antigens based on coagulation and perform  ELISA, Ouchterlony double diffusion.

The approach in teaching:

Interactive Lectures, Discussion, Tutorials, Reading  assignments, Demonstration,

Learning activities for the  students:

Self-learning assignments,Effective questions, Simulation, Seminar presentation, Giving tasks, Practical skills and laboratory reports

Lab demonstration Class test Semester end  examination s Quiz Solving  problems in tutorials Assignments  Presentation Individual and  group projects  Viva voce
 
 
Biotechnology and Microbiology: 
 
● Principle and working of equipment’s used in biotechnology laboratory (SDS-PAGE  apparatus, horizontal DNA Electrophoresis chamber, Laminar Airflow, Autoclave, Incubator,  pH meter, Homogenizer, Ascending and Descending Chromatographic chamber, Thin Layer  Chromatographic Apparatus, Microtome) 
● Preparation and use culture media for microbes 
● Study of microbes in food material (curd, milk, rotten fruits and vegetables) 
● Educational tour to any Microbiology lab (Food processing factory, Distillery, Dairy etc.) Candidates will have to submit a report on it. 
● Culturing of Bacteria from soil/water samples. 
 
Immunology: 
● Experiments on Antigen and Antibody reaction. 
● Blood typing 
● ELISA 
● Ouchterlony double diffusion 
 
Essential Readings: 
ESSENTIAL READINGS:
● Practical Biochemistry: Principles & Techniques; Keith Wilson& John Walker;  Cambridge University Press. 
● Microbiology, A laboratory manual by Cappuccino, James,G. 
● Lab Manual of microbiology & biotechnology by K. R. Aneja. Scientific international private  limited
 
SUGGESTED READINGS:
● Roitt’s essential Immunology, P. Delves, et al., 11th ed., Blackwell Publishing, 2006
● Kindt, T. J., Goldsby, R.A., Osborne, B. A. and Kuby, J (2006). Immunology, VI Edition.  W.H. Freeman and  Company. 
● Molecular Biotechnology. Glick B.R. & Pasternak J. J; ASM Press.
● David, M., Jonathan, B., David, R. B. and Ivan R. (2006). Immunology,VII Edition, Mosby,  Elsevier Publication. 
● Abbas, K. Abul and Lechtman H. Andrew (2017) Cellular and Molecular Immunology. V  Edition. Elsevier; 9th edition 
 
e-RESOURCES:
 
Academic Year: 
2022-2023
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