Practical

Paper Code: 
ZOL-215
Credits: 
4
Contact Hours: 
120.00
Max. Marks: 
100.00
Objective: 
Course objectives:  
This course will enable the students to - 
1. Analyze invertebrate laboratory specimens based on their external features and appearance  
2. Implement molecular biology techniques including gel electrophoresis and chromatography  and their applications in the research field. 
3. Differentiate wild and mutant strains of Drosophila. 
4. Perform qualitative and quantitative biochemical techniques to differentiate between different  biomolecules based on their physical and chemical properties. 
5. Analyze different modes of central tendency, standard deviation and variance.
 
Course Outcomes (COs): 
 

Course 

Learning outcomes 

(at course level)

Learning and  teaching strategies

Assessment  Strategies 

Paper  

Code

Paper Title

ZOL-215 

Practical 

The students will: 

CO 41: Identify different laboratory  specimens based on their external  features and appearance and their  categorical relation with various  phylum. 

CO 42: Get hands-on experience of  various molecular biology techniques  including gel electrophoresis and  chromatography and would learn  about their applications in the research  field. 

CO 43: Enhance their knowledge  about wild and mutant strains of  Drosophila and their important  structures. 

CO 44: Apply qualitative and  quantitative biochemical techniques to  differentiate between different  biomolecules based on their physical  and chemical properties. 

CO 45: Perform calculations of  different modes of central tendency,  standard deviation and will be able to  calculate variance.

The approach in  teaching: 

Interactive Lectures,  Discussion, Tutorials,  Reading assignments,  Demonstration, Team  teaching 

Learning activities for  the students: 

Self-learning  

assignments, Effective  questions, Simulation,  Seminar presentation,  Giving tasks, Field  practical, demonstration  by virtual software.

Class test  

Semester end  examinations, Quiz,  

Assignments,  Presentation 

Surprise test 

Open book test

 

Biochemistry II 
  • Colorimetric/Spectrophotometric estimation of: 
  • Carbohydrate-Glucose
  • Preparation of Phosphate buffer 
  • Isolation of protein (casein) from milk 
  • Study of temperature effect on enzyme action: Salivary amylase 
 
Animal Diversity III 
  • Study of museum specimens and slides: 
  • Phylum Annelida-Nereis, Heteronereis-parapodia, Earthworm, Leech 
  • Dissection (Demonstration): Earthworm- Anatomy, Digestive, Nervous System Permanent mount:Septalnephridia, Ovary,Nerve ring. 
  • Phylum Arthropoda: Daphnia, CyCOps, Scorpion, King Crab, Crab, Eupagurus, Balanus, Millipede,  Termite, Centipede, Praying-mantis, Peripatus
  • Dissection: (Demonstration): Prawn-General anatomy, Appendages, Nervous & Digestive system Permanent mount: Hastate plate, Statocyst 
  • Phylum-Mollusca:Chiton, Unio, Dentalium, Nautilus, Loligo, Octopus, Sepia 
  • Phylum Echinodermata: Echinus, Starfish, Cucumaria, Antedon
  • Larval forms- Miracidium, Sporocyst, Redia, Cercaria,Nauplius,Zoea,Mysis,cypris,Megalopa,  Glochidium larva. 
 
Molecular Biology: 
  • DNA isolation and separation by Agarose gel method 
  • Vertical and horizontal electrophoresis unit (demonstration only) 
  • Paper chromatographic separation of amino acids 
 
Genetics &Biostatistics:
  • Study of Drosophila life cycle & idea about its culture 
  • Identification of Male & Female Drosophila 
  • Identification of Wild & mutant (Yellow body, Ebony, Vestigial wings, White eye, Apterus) of  Drosophila 
  •  Study of permanent prepared slide - Sex comb, Salivary gland chromosomes
  • Identification of Blood groups (A,B, O & Rh factor)
  • Preparation of frequency tables and graphs manually and with help of computers.
  • Calculation of Mean, Median, Mode
  • Calculation of standard deviation, variance and standard error of the mean.
  • Chi square test for independence of attributes, t test
 
 
Essential Readings: 
● Vertebrates Comparative anatomy, function, evolution, K.V. Kardong, Tata-McGraw  Hill,New Delhi. 
● Practical Zoology, vertebrates, Lal, S.S. Rastogi Publications, Meerut 
● Practical Zoology, vertebrates,Kohli,K.S.,Ansari, A.S. Ramesh Book Depot, Jaipur
● Introductory practical Biochemistry, Sawhney S.K., Singh R. 2009. 7th edition, Narosa publishing house Pvt. Ltd.
● Wilson,K. and Walker, J. Principles and techniques of practical biochemistry,  Cambridge press.
● Jordon, E.L., Verma P.S. Invertebrate Zoology, S Chand and Co.
● Lboratory manual in Invertebrate & Experimental Zoology, Jacob VyasRamesh Book Depot, New Delhi.
● Biostatistics; Gupta, S.P.;Sultan Chand & Co
● Practical Exercises in Cytology, Genetics, Plant Breeding and Biostatistics. Raghuvanshi.
 
References: 
SUGGESTED READINGS:
● Zubay G. Biochemistry, William C. Brown Pub. 
● Standard methods of biochemical analysis.Thimmaiah, S.R. KalyaniPublishers,Noida
 
e-RESOURCES: 
● Experimental Biochemistry- NPTEL- https://nptel.ac.in/courses/104105102
● Biochemistry virtual lab- Amrita- https://vlab.amrita.edu/?sub=3&brch=64 
 
Academic Year: 
2022-2023
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